Toward metabolic engineering in the context of system biology and synthetic biology: advances and prospects
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Metabolic engineering facilitates the rational development of recombinant bacterial strains for metabolite overproduction. Building on enormous advances in system biology and synthetic biology, novel strategies have been established for multivariate optimization of metabolic networks in ensemble, spatial, and dynamic manners such as modular pathway engineering, compartmentalization metabolic engineering, and metabolic engineering guided by genome-scale metabolic models, in vitro reconstitution, and systems and synthetic biology. Herein, we summarize recent advances in novel metabolic engineering strategies. Combined with advancing kinetic models and synthetic biology tools, more efficient new strategies for improving cellular properties can be established and applied for industrially important biochemical production.
KeywordsSystem metabolic engineering Modular pathway engineering Synthetic biology Genome-scale metabolic model Spatial engineering
This work was financially supported by the Enterprise-university-research prospective program, Jiangsu Province (BY2012054), 111 Project (111-2-06), and 973 project (2012CB720806). We are also thankful for the constructive advice of Prof. Uwe Sauer from ETH Zürich.
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