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Recent research progress on microbial l-asparaginases

Abstract

l-Asparaginases (EC 3.5.1.1) are enzymes that catalyze the hydrolysis of l-asparagine to l-aspartic acid and found in a variety of organisms from microorganisms to mammals. However, they are mainly expressed and produced by microorganisms. Microbial l-asparaginases have received sustained attention due to their irreplaceable role in the therapy of acute lymphoblastic leukemia and for their inhibition of acrylamide formation during food processing. In this article, we review the application of microbial l-asparaginases in medical treatments and acrylamide mitigation. In addition, we describe in detail recent advances in the existing sources, purification, production, properties, molecular modification, and immobilization of l-asparaginase.

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Acknowledgments

This work was supported by the 973 Project (No. 2012CB720802), the 863 Project (No. 2011AA100904), and the Support Project of Jiangsu Province (No. BK20130001) and Shaoxing City (No. 2013A23002).

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Correspondence to Wanmeng Mu.

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Zuo, S., Zhang, T., Jiang, B. et al. Recent research progress on microbial l-asparaginases. Appl Microbiol Biotechnol 99, 1069–1079 (2015). https://doi.org/10.1007/s00253-014-6271-9

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  • DOI: https://doi.org/10.1007/s00253-014-6271-9

Keywords

  • l-Asparaginase
  • Acute lymphoblastic leukemia
  • Acrylamide
  • Production
  • Modification
  • Immobilization