Abstract
Selective capture of transcribed sequences (SCOTS) is an effective method to identify bacterial genes differentially expressed during different biological processes, including pathogenic interactions with a host species. The method can be used to elucidate molecular mechanisms driving and maintaining such interactions. The method is a powerful genetic tool that overcomes limitations found in other methods, by working with small amounts of mRNA and allowing for the separation of bacterial cDNA from host cDNA. It has been increasingly used in the discovery of genes involved in the bacterium-host interaction. In this review, we briefly introduce the SCOTS method, outline the technical advances offered in the method, and focus on the method’s applications in several microbial pathogens.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31201910), Sponsored by the Program for Science & Technology Innovation Talents in Universities of Henan Province (14HASTIT024), and Foundation for University Key Teacher by the Ministry of Education of Henan Province (2013GGJS-068), the Science and Technology development project of Henan Province (142102310297), the Science and Technology Research Foundation of Henan Province Educational Committee (13A230261, 14A230003).
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Wang, Y., Yi, L., Wang, S. et al. Selective capture of transcribed sequences in the functional gene analysis of microbial pathogens. Appl Microbiol Biotechnol 98, 9983–9992 (2014). https://doi.org/10.1007/s00253-014-6190-9
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DOI: https://doi.org/10.1007/s00253-014-6190-9