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Comparative proteomics reveal the impact of OmcA/MtrC deletion on Shewanella oneidensis MR-1 in response to hexavalent chromium exposure

  • Genomics, transcriptomics, proteomics
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Hexavalent chromium [Cr(VI)] is a priority pollutant causing serious environmental issues. Microbial reduction provides an alternative strategy for Cr(VI) remediation. The dissimilatory metal-reducing bacterium, Shewanella oneidensis MR-1, was employed to study Cr(VI) reduction and toxicity in this work. To understand the effect of membrane cytochromes on Cr(VI) response, a comparative protein profile analysis from S. oneidensis MR-1 wild type and its mutant of deleting OmcA and MtrC (△omcA/mtrC) was conducted using two-dimensional electrophoresis (2-DE) technology. The 2-DE patterns were compared, and the proteins with abundant changes of up to twofold in the Cr(VI) treatment were detected. Using mass spectrometry, 38 and 45 differentially abundant proteins were identified in the wild type and the mutant, respectively. Among them, 25 proteins were shared by the two strains. The biological functions of these identified proteins were analyzed. Results showed that Cr(VI) exposure decreased the abundance of proteins involved in transcription, translation, pyruvate metabolism, energy production, and function of cellular membrane in both strains. There were also significant differences in protein expressions between the two strains under Cr(VI) treatment. Our results suggest that OmcA/MtrC deletion might result in the Cr(VI) toxicity to outer membrane and decrease assimilation of lactate, vitamin B12, and cystine. When carbohydrate metabolism was inhibited by Cr(VI), leucine and sulfur metabolism may act as the important compensatory mechanisms in the mutant. Furthermore, the mutant may regulate electron transfer in the inner membrane and periplasm to compensate for the deletion of OmcA and MtrC in Cr(VI) reduction.

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Acknowledgments

This research was supported financially by the National Natural Science Foundation of China (21322703, 21177122), China Postdoctoral Science Foundations (2012M520411), and Science and Technology Innovation and Collaboration Team Project of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China

    Chao Wang & Feng Zhao

  2. Key Laboratory of the Coastal and Wetland Ecosystems, Ministry of Education, College of the Environment and Ecology, Xiamen University, Xiamen, 361005, China

    Juan Chen, Wen-Jun Hu, Ji-Yun Liu & Hai-Lei Zheng

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  1. Chao Wang
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  2. Juan Chen
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  3. Wen-Jun Hu
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  4. Ji-Yun Liu
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  5. Hai-Lei Zheng
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  6. Feng Zhao
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Correspondence to Feng Zhao.

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Chao Wang and Juan Chen contributed equally to this work.

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Wang, C., Chen, J., Hu, WJ. et al. Comparative proteomics reveal the impact of OmcA/MtrC deletion on Shewanella oneidensis MR-1 in response to hexavalent chromium exposure. Appl Microbiol Biotechnol 98, 9735–9747 (2014). https://doi.org/10.1007/s00253-014-6143-3

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  • DOI: https://doi.org/10.1007/s00253-014-6143-3

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