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Transcriptome-guided identification of SprA as a pleiotropic regulator in Streptomyces chattanoogensis

Applied Microbiology and Biotechnology volume 99, pages 1287–1298 (2015)Cite this article

Abstract

Quorum sensing molecular γ-butyrolactones (GBL) are widely distributed among the genus Streptomyces. Their cognate receptors have been demonstrated to control secondary metabolism and/or morphological differentiation. ScgA is responsible for the biosynthesis of GBL in Streptomyces chattanoogensis. According to the genome-wide transcriptome analysis of the ΔscgA mutant, we found that the expression of sprA, which encodes a GBL receptor homologue, was shown to be positively regulated by ScgA. Electrophoretic mobility shift assays and DNase I footprinting assays showed that SprA bound to two specific autoregulatory element (ARE) sequences located upstream of the sprA gene, indicating that its expression is self-regulated. SprA was involved in biosynthesis of GBL by repressing the expression of scgA. An Escherichia coli-based luciferase report system demonstrated that SprA directly repressed the expression of scgR, which encodes a GBL receptor. Like deletion of scgA, the disruption of sprA resulted in decreased production of the antibiotic natamycin in liquid culture and retarded morphological differentiation on solid agar. This work indicates that SprA acts as a pleiotropic regulator of both morphogenesis and the production of natamycin.

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Acknowledgments

This work was supported by Key Program of Zhejiang Provincial Natural Science Foundation of China (LZ12C01001), National High Technology Research and Development Program of China (863 Program, 2012AA02A706), National Basic Research Program of China (973 Program, 2012CB721005), and Specialized Research Fund for the Doctoral Program of Higher Education (No. 20120101110143).

We sincerely thank Professor Ke-Qian Yang (Institute of Microbiology, Chinese Academy of Science) for providing the plasmids pACYC184 and pCS26-pac. We are grateful to Professor Qing-Qing Wang (School of Basic Medical Sciences, Zhejiang University) for measuring luciferase. We deeply appreciate the critical advice of Dr. Yi-Ling Du on this work.

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Authors and Affiliations

  1. Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China

    Zhen-Xing Zhou, Qing-Qing Xu, Qing-Ting Bu, Shui-Ping Liu, Pin Yu & Yong-Quan Li

  2. Zhejiang Provincial Key Laboratory of Microbial Biochemistry and Metabolism Engineering, Hangzhou, 310058, China

    Yong-Quan Li

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  1. Zhen-Xing Zhou
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Correspondence to Yong-Quan Li.

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Zhou, ZX., Xu, QQ., Bu, QT. et al. Transcriptome-guided identification of SprA as a pleiotropic regulator in Streptomyces chattanoogensis . Appl Microbiol Biotechnol 99, 1287–1298 (2015). https://doi.org/10.1007/s00253-014-6132-6

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