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Synergistic proteins for the enhanced enzymatic hydrolysis of cellulose by cellulase

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Abstract

Reducing the enzyme loadings for enzymatic saccharification of lignocellulose is required for economically feasible production of biofuels and biochemicals. One strategy is addition of small amounts of synergistic proteins to cellulase mixtures. Synergistic proteins increase the activity of cellulase without causing significant hydrolysis of cellulose. Synergistic proteins exert their activity by inducing structural modifications in cellulose. Recently, synergistic proteins from various biological sources, including bacteria, fungi, and plants, were identified based on genomic data, and their synergistic activities were investigated. Currently, an up-to-date overview of several aspects of synergistic proteins, such as their functions, action mechanisms and synergistic activity, are important for future industrial application. In this review, we summarize the current state of research on four synergistic proteins: carbohydrate-binding modules, plant expansins, expansin-like proteins, and Auxiliary Activity family 9 (formerly GH61) proteins. This review provides critical information to aid in promoting research on the development of efficient and industrially feasible synergistic proteins.

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Acknowledgments

This work was supported by grants from the National Research Foundation of Korea (2013M1A2A2072597) and the Advanced Biomass R&D Center of Korea (2011-0031353), both funded by the Korean Government (MSIP). This research was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.

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  1. Department of Biotechnology, Korea University Graduate School, Seoul, 136-713, Republic of Korea

    In Jung Kim, Hee Jin Lee, In-Geol Choi & Kyoung Heon Kim

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  1. In Jung Kim
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  2. Hee Jin Lee
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  3. In-Geol Choi
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  4. Kyoung Heon Kim
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Correspondence to Kyoung Heon Kim.

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Kim, I.J., Lee, H.J., Choi, IG. et al. Synergistic proteins for the enhanced enzymatic hydrolysis of cellulose by cellulase. Appl Microbiol Biotechnol 98, 8469–8480 (2014). https://doi.org/10.1007/s00253-014-6001-3

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  • DOI: https://doi.org/10.1007/s00253-014-6001-3

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