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Simultaneous saccharification and fermentation of solid household waste following mild pretreatment using a mix of hydrolytic enzymes in combination with Saccharomyces cerevisiae


Ethanol production from low severity pretreated (85 °C, 1 h) solid household waste was studied using simultaneous saccharification and fermentation (SSF). The aim of the study was to examine typical composition of the organic fraction of municipal solid waste (OFMSW) and to develop a simple method for simultaneous liquefaction and biofuels production. A model waste was prepared based on the composition of the organic waste in Masdar City. Chemical analysis of the OFMSW showed that it contained 37 % total solids with up to 57 g glucan/100 g total solid (TS). Hydrolysis of the wet OFMSW was carried out using a mix of hydrolytic enzymes: amylase, cellulase, protease, lipase, hemicellulase, and pectate lyase. The enzymatic hydrolysis using this enzyme mix was studied using different dilutions of the OFMSW at different enzyme loadings. This study has demonstrated that SSF of low severity pretreated OFMSW can be carried out using Saccharomyces cerevisiae without dilution (addition of water), and liquefaction of the undiluted OFMSW can be achieved in less than 24 h of hydrolysis. Also, SSF of the pretreated waste can be carried out with very low enzyme loading (10 % of the company recommended dosage)—0.1 % cellulase, 0.1 % amylase, 0.02 % protease, 0.02 % hemicellulase, 0.02 % lipase, and 0.02 % pectate lyase (w/w per TS) following mild heat pretreatment conditions of 85 °C for 1 h.

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  1. Abdel-Rahman MA, Tashiro Y, Sonomoto K (2011) Lactic acid production from lignocellulose-derived sugars using lactic acid bacteria: overview and limits. J Biotechnol 156:286–301

  2. Al Ashram O (2008) Wastes and pollution sources of Abu Dhabi Emirate, UAE. Environmental Agency, Abu Dhabi, UAE

  3. Balat M (2011) Production of bioethanol from lignocellulosic materials via the biochemical pathway. Energ Convers Manag 52:858–875

  4. Cui F, Li YL, Wan C (2011) Lactic acid production from corn stover using mixed cultures of Lactobacillus rhamnosus and Lactobacillus brevis. Bioresour Technol 102:1831–1836

  5. Curry N, Pillay P (2011) Biogas prediction and design of a food waste to energy system for the urban environment. Renew Energ 1–10

  6. Demirbas A (2010) Biofuels from biomass. In Biorefineries: For biomass upgrading facilities (pp. 34–73). Springer

  7. Demirbas FM, Balat M, Balat H (2011) Biowastes-to-biofuels. Energ Convers Manag 10(041):1815–1828

  8. Guadalupe G, Montserrat M, Lourdes B, Francesc C (2009) Seasonal characterization of municipal solid waste (MSW) in the city of Chihuahua, Mexico. Waste Manag 02(006):2018–2024

  9. OECD Environmental Performance and Information Division (2007) OECD Environmental Data, COMPENDIUM 2006–2008. Working Group on Environmental Information and Outlooks, OCED

  10. Jacques KA, Lyons TP, Kelsall DK (2003) The alcohol textbook. Nottingham University Press, Nottingham

  11. Jayasinghe P, Hettiaratchi J, Mehrotra A, Kumar S (2011) Effect of enzyme additions on methane production and lignin degradation of landfilled sample of municipal solid waste. Bioresour Technol 101:4633–4637

  12. Jensen JW, Felby C, Jørgensen H, Rønsch GØ, Nørholm ND (2010) Enzymatic processing of municipal solid waste. Waste Manag 30:2497–2503

  13. John RP, Anisha GS, Nampoothiri KM, Pandey A (2009) Direct lactic acid fermentation: focus on simultaneous saccharification and lactic acid production. Biotechnol Adv 27:145–152

  14. Jørgensen H, Vibe-Pedersen J, Larsen J, Felby C (2006) Liquefaction of lignocellulose at high-solids concentrations. Biotechnol Bioeng 96(5):862–870

  15. Kaparaju P, Serrano M, Thomsen AB, Kongjan P, Angelidaki I (2009) Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept. Bioresour Technol 100:2562–2568

  16. Kim JH, Lee JC, Pak D (2011) Feasibility of producing ethanol from food waste. Waste Manag 31:2121–2125

  17. Kretschmer B, Allen B, Kieve D, Smith C (2013) Shifting away from conventional biofuels: sustainable alternatives for the use of biomass in the UK transport sector. Institute for European Environmental Policy (IEEP), London

  18. Lin Y, Tanaka S (2006) Ethanol fermentation from biomass resources: current state and prospects. Appl Microbiol Biotechnol 69:627–642

  19. Matsakas L, Kekos D, Loizidou M and Christakopoulos P (2014) Utilization of household food waste for the production of ethanol at high dry material content. Biotechnol Biofuels, 7

  20. Meor Hussin AS, Collins SRA, Merali Z, Parker ML, Elliston A, Wellner N, Waldron KW (2013) Characterisation of lignocellulosic sugars from municipal solid waste residue. Biomass Bioenergy 51:17–25

  21. Ohgren K, Bura R, Lesnick G, Saddler J, Zacchi G (2007) A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover. Process Biochem 42:834–839

  22. Sluiter A, Crocker D, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008a) Determination of structural carbohydrates and lignin in biomass. NREL

  23. Sluiter A, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008b) Determination of extractives in biomass. NREL, Colorado

  24. Solid Waste and Emergency Response, EPA US (2013) Municipal solid waste generation, recycling, and disposal in the United States: facts and figures for 2011. US Environmental Protection Agency, Washington

  25. Statistics center, Abu Dhabi (2011) Statistical yearbook of Abu Dhabi 2011

  26. Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83:1–11

  27. Taherzadeh MJ, Karimi K (2007) Enzyme-based hydrolysis processes for ethanol from lignocellulosic materials: a review. Bio Res 2(4):707–738

  28. The Center of Waste Management Abu Dhabi (2010) NADAFA program. Forum quarterly meeting. Abu Dhabi Sustainability Group, Abu Dhabi

  29. Troschinetz AM, Mihelcic JR (2009) Sustainable recycling of municipal solid waste in developing countries. Waste Manag 29:915–923

  30. Weiss N, Börjesson J, Pedersen LS, Meyer AS (2013) Enzymatic lignocellulose hydrolysis: improved cellulase productivity by insoluble solids recycling. Biotechnol Biofuels 6

  31. Zhang DQ, Tan SK, Gersberg RM (2010) Municipal solid waste management in China: status, problems and challenges. J Environ Manag 91:1623–1633

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The support of the Government of Abu Dhabi through Masdar Institute of Science and Technology is greatly appreciated.

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Correspondence to M. H. Thomsen.

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Nwobi, A., Cybulska, I., Tesfai, W. et al. Simultaneous saccharification and fermentation of solid household waste following mild pretreatment using a mix of hydrolytic enzymes in combination with Saccharomyces cerevisiae . Appl Microbiol Biotechnol 99, 929–938 (2015). https://doi.org/10.1007/s00253-014-5977-z

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  • Enzyme
  • Ethanol
  • Hydrolysis
  • Fermentation
  • Waste