Applied Microbiology and Biotechnology

, Volume 99, Issue 2, pp 929–938 | Cite as

Simultaneous saccharification and fermentation of solid household waste following mild pretreatment using a mix of hydrolytic enzymes in combination with Saccharomyces cerevisiae

  • A. Nwobi
  • I. Cybulska
  • W. Tesfai
  • Y. Shatilla
  • J. Rodríguez
  • M. H. Thomsen
Environmental biotechnology

Abstract

Ethanol production from low severity pretreated (85 °C, 1 h) solid household waste was studied using simultaneous saccharification and fermentation (SSF). The aim of the study was to examine typical composition of the organic fraction of municipal solid waste (OFMSW) and to develop a simple method for simultaneous liquefaction and biofuels production. A model waste was prepared based on the composition of the organic waste in Masdar City. Chemical analysis of the OFMSW showed that it contained 37 % total solids with up to 57 g glucan/100 g total solid (TS). Hydrolysis of the wet OFMSW was carried out using a mix of hydrolytic enzymes: amylase, cellulase, protease, lipase, hemicellulase, and pectate lyase. The enzymatic hydrolysis using this enzyme mix was studied using different dilutions of the OFMSW at different enzyme loadings. This study has demonstrated that SSF of low severity pretreated OFMSW can be carried out using Saccharomyces cerevisiae without dilution (addition of water), and liquefaction of the undiluted OFMSW can be achieved in less than 24 h of hydrolysis. Also, SSF of the pretreated waste can be carried out with very low enzyme loading (10 % of the company recommended dosage)—0.1 % cellulase, 0.1 % amylase, 0.02 % protease, 0.02 % hemicellulase, 0.02 % lipase, and 0.02 % pectate lyase (w/w per TS) following mild heat pretreatment conditions of 85 °C for 1 h.

Keywords

Enzyme Ethanol Hydrolysis Fermentation Waste 

References

  1. Abdel-Rahman MA, Tashiro Y, Sonomoto K (2011) Lactic acid production from lignocellulose-derived sugars using lactic acid bacteria: overview and limits. J Biotechnol 156:286–301CrossRefPubMedGoogle Scholar
  2. Al Ashram O (2008) Wastes and pollution sources of Abu Dhabi Emirate, UAE. Environmental Agency, Abu Dhabi, UAEGoogle Scholar
  3. Balat M (2011) Production of bioethanol from lignocellulosic materials via the biochemical pathway. Energ Convers Manag 52:858–875CrossRefGoogle Scholar
  4. Cui F, Li YL, Wan C (2011) Lactic acid production from corn stover using mixed cultures of Lactobacillus rhamnosus and Lactobacillus brevis. Bioresour Technol 102:1831–1836CrossRefPubMedGoogle Scholar
  5. Curry N, Pillay P (2011) Biogas prediction and design of a food waste to energy system for the urban environment. Renew Energ 1–10Google Scholar
  6. Demirbas A (2010) Biofuels from biomass. In Biorefineries: For biomass upgrading facilities (pp. 34–73). SpringerGoogle Scholar
  7. Demirbas FM, Balat M, Balat H (2011) Biowastes-to-biofuels. Energ Convers Manag 10(041):1815–1828CrossRefGoogle Scholar
  8. Guadalupe G, Montserrat M, Lourdes B, Francesc C (2009) Seasonal characterization of municipal solid waste (MSW) in the city of Chihuahua, Mexico. Waste Manag 02(006):2018–2024Google Scholar
  9. OECD Environmental Performance and Information Division (2007) OECD Environmental Data, COMPENDIUM 2006–2008. Working Group on Environmental Information and Outlooks, OCEDGoogle Scholar
  10. Jacques KA, Lyons TP, Kelsall DK (2003) The alcohol textbook. Nottingham University Press, NottinghamGoogle Scholar
  11. Jayasinghe P, Hettiaratchi J, Mehrotra A, Kumar S (2011) Effect of enzyme additions on methane production and lignin degradation of landfilled sample of municipal solid waste. Bioresour Technol 101:4633–4637CrossRefGoogle Scholar
  12. Jensen JW, Felby C, Jørgensen H, Rønsch GØ, Nørholm ND (2010) Enzymatic processing of municipal solid waste. Waste Manag 30:2497–2503CrossRefPubMedGoogle Scholar
  13. John RP, Anisha GS, Nampoothiri KM, Pandey A (2009) Direct lactic acid fermentation: focus on simultaneous saccharification and lactic acid production. Biotechnol Adv 27:145–152CrossRefPubMedGoogle Scholar
  14. Jørgensen H, Vibe-Pedersen J, Larsen J, Felby C (2006) Liquefaction of lignocellulose at high-solids concentrations. Biotechnol Bioeng 96(5):862–870CrossRefGoogle Scholar
  15. Kaparaju P, Serrano M, Thomsen AB, Kongjan P, Angelidaki I (2009) Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept. Bioresour Technol 100:2562–2568CrossRefPubMedGoogle Scholar
  16. Kim JH, Lee JC, Pak D (2011) Feasibility of producing ethanol from food waste. Waste Manag 31:2121–2125CrossRefPubMedGoogle Scholar
  17. Kretschmer B, Allen B, Kieve D, Smith C (2013) Shifting away from conventional biofuels: sustainable alternatives for the use of biomass in the UK transport sector. Institute for European Environmental Policy (IEEP), LondonGoogle Scholar
  18. Lin Y, Tanaka S (2006) Ethanol fermentation from biomass resources: current state and prospects. Appl Microbiol Biotechnol 69:627–642CrossRefPubMedGoogle Scholar
  19. Matsakas L, Kekos D, Loizidou M and Christakopoulos P (2014) Utilization of household food waste for the production of ethanol at high dry material content. Biotechnol Biofuels, 7Google Scholar
  20. Meor Hussin AS, Collins SRA, Merali Z, Parker ML, Elliston A, Wellner N, Waldron KW (2013) Characterisation of lignocellulosic sugars from municipal solid waste residue. Biomass Bioenergy 51:17–25CrossRefGoogle Scholar
  21. Ohgren K, Bura R, Lesnick G, Saddler J, Zacchi G (2007) A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover. Process Biochem 42:834–839CrossRefGoogle Scholar
  22. Sluiter A, Crocker D, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008a) Determination of structural carbohydrates and lignin in biomass. NRELGoogle Scholar
  23. Sluiter A, Ruiz R, Scarlata C, Sluiter J, Templeton D (2008b) Determination of extractives in biomass. NREL, ColoradoGoogle Scholar
  24. Solid Waste and Emergency Response, EPA US (2013) Municipal solid waste generation, recycling, and disposal in the United States: facts and figures for 2011. US Environmental Protection Agency, WashingtonGoogle Scholar
  25. Statistics center, Abu Dhabi (2011) Statistical yearbook of Abu Dhabi 2011Google Scholar
  26. Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresour Technol 83:1–11CrossRefPubMedGoogle Scholar
  27. Taherzadeh MJ, Karimi K (2007) Enzyme-based hydrolysis processes for ethanol from lignocellulosic materials: a review. Bio Res 2(4):707–738Google Scholar
  28. The Center of Waste Management Abu Dhabi (2010) NADAFA program. Forum quarterly meeting. Abu Dhabi Sustainability Group, Abu DhabiGoogle Scholar
  29. Troschinetz AM, Mihelcic JR (2009) Sustainable recycling of municipal solid waste in developing countries. Waste Manag 29:915–923CrossRefPubMedGoogle Scholar
  30. Weiss N, Börjesson J, Pedersen LS, Meyer AS (2013) Enzymatic lignocellulose hydrolysis: improved cellulase productivity by insoluble solids recycling. Biotechnol Biofuels 6Google Scholar
  31. Zhang DQ, Tan SK, Gersberg RM (2010) Municipal solid waste management in China: status, problems and challenges. J Environ Manag 91:1623–1633CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • A. Nwobi
    • 1
  • I. Cybulska
    • 1
  • W. Tesfai
    • 1
  • Y. Shatilla
    • 1
  • J. Rodríguez
    • 2
  • M. H. Thomsen
    • 1
  1. 1.Institute Center for Energy – iEnergyMasdar Institute of Science and TechnologyAbu DhabiUnited Arab Emirates
  2. 2.Institute Centre for Water and Environment - iWaterMasdar Institute of Science and technologyAbu DhabiUnited Arab Emirates

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