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Bromelain: an overview of industrial application and purification strategies

Applied Microbiology and Biotechnology volume 98, pages 7283–7297 (2014)Cite this article

Abstract

This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.

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Authors and Affiliations

  1. Bioprocess and Molecular Engineering Research Unit, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia

    Zatul Iffah Mohd Arshad, Azura Amid, Faridah Yusof & Irwandi Jaswir

  2. Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300, Gambang, Kuantan, Pahang, Malaysia

    Zatul Iffah Mohd Arshad

  3. Kuliyyah of Pharmacy, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia

    Kausar Ahmad

  4. Manufacturing and Industrial Processes Division, Faculty of Engineering, Centre for Food and Bioproduct Processing, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, 43500, Selangor Darul Ehsan, Malaysia

    Show Pau Loke

Authors
  1. Zatul Iffah Mohd Arshad
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  2. Azura Amid
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  3. Faridah Yusof
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  4. Irwandi Jaswir
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  5. Kausar Ahmad
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Correspondence to Azura Amid.

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Arshad, Z.I.M., Amid, A., Yusof, F. et al. Bromelain: an overview of industrial application and purification strategies. Appl Microbiol Biotechnol 98, 7283–7297 (2014). https://doi.org/10.1007/s00253-014-5889-y

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  • DOI: https://doi.org/10.1007/s00253-014-5889-y

Keywords

  • Bromelain
  • Cysteine protease
  • Purification
  • Recombinant bromelain