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Proteomics of arsenic stress in the gram-positive organism Exiguobacterium sp. PS NCIM 5463

  • Genomics, transcriptomics, proteomics
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Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The general responses of microorganisms to environmental onslaughts are modulated by altering the gene expression pattern to reduce damage in the cell and produce compensating stress responses. The present study attempts to unravel the response of the Gram-positive Exiguobacterium sp. PS NCIM 5463 in the presence of [As(III)] and arsenate [As(V)] using comparative proteomics via two-dimension gel electrophoresis (2-DE) coupled with identification of proteins using matrix-assisted laser desorption/ionisation (MALDI-TOF/MALDI-TOF/TOF). Out of 926 Coomassie-stained proteins, 45 were differentially expressed (p < 0.05). Considering the resolution and abundance level, 24 spots (peptides) were subjected to MALDI analysis, identified and categorised into several functional categories, viz., nitrogen metabolism, energy and stress regulators, carbohydrate metabolism, protein synthesis components and others. A functional role of each protein is discussed in Exiguobacterium sp. PS 5463 under arsenic stress and validated at their transcript level using a quantitative real-time polymerase chain reaction. Unlike previous reports that unravel the responses toward arsenic stress in Gram-negative organisms, the present study identified new proteins under arsenic stress in a Gram-positive organism, Exiguobacterium sp. PS NCIM 5463, which could elucidate the physiology of organisms under arsenic stress.

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Acknowledgments

The authors are thankful to funding agencies, viz., Biotechnology Departmental Research and Development Grant, University of Pune, DST-PURSE Programme, Delhi, and CSIR-SRF fellowship grant to PS, and UPE-II Phase programme for financial assistance.

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Authors and Affiliations

  1. Department of Biotechnology, University of Pune, Ganeshkhind, Pune, 411007, India

    Poonam Sacheti, Rajendra Patil, Ankita Dube, Dipalee Thombre, Sayali Marathe, Ravindra Vidhate, Priyanka Wagh & Wasudev Gade

  2. Proteomics Facility, Division of Biochemistry, CSIR-National Chemical Laboratory, Pune, 411008, India

    Hemangi Bhonsle & Mahesh Kulkarni

  3. Proteomics Lab, National Centre for Cell Science, Pune, 411007, India

    Srikanth Rapole

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  1. Poonam Sacheti
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  2. Rajendra Patil
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Correspondence to Wasudev Gade.

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Sacheti, P., Patil, R., Dube, A. et al. Proteomics of arsenic stress in the gram-positive organism Exiguobacterium sp. PS NCIM 5463. Appl Microbiol Biotechnol 98, 6761–6773 (2014). https://doi.org/10.1007/s00253-014-5873-6

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