Advertisement

Applied Microbiology and Biotechnology

, Volume 98, Issue 13, pp 6051–6060 | Cite as

Influence of intrapartum antibiotic prophylaxis against group B Streptococcus on the early newborn gut composition and evaluation of the anti-Streptococcus activity of Bifidobacterium strains

  • Irene Aloisio
  • Giuseppe Mazzola
  • Luigi Tommaso Corvaglia
  • Giacomo Tonti
  • Giacomo Faldella
  • Bruno Biavati
  • Diana Di GioiaEmail author
Applied microbial and cell physiology

Abstract

Several factors are known to influence the early colonization of the gut in newborns. Among them, the use of antibiotics on the mother during labor, referred to as intrapartum antibiotic prophylaxis (IAP), has scarcely been investigated, although this practice is routinely used in group B Streptococcus (GBS)-positive women. This work is therefore aimed at verifying whether IAP can influence the main microbial groups of the newborn gut microbiota at an early stage of microbial establishment. Fifty-two newborns were recruited: 26 born by mothers negative to GBS (control group) and 26 by mothers positive to GBS and subjected to IAP with ampicillin (IAP group). Selected microbial groups (Lactobacillus spp., Bidobacterium spp., Bacteroides fragilis, Clostridium difficile, and Escherichia coli) were quantified with real-time PCR on DNA extracted from newborn feces. Further analysis was performed within the Bidobacterium genus by using DGGE after amplification with genus-specific primers. Results obtained showed a significant decrease of the bifidobacteria counts after antibiotic treatment of the mother. Bifidobacteria were found to be affected by IAP not only quantitatively but also qualitatively. In fact, IAP determined a decrement in the frequency of Bidobacterium breve, Bidobacterium bifidum, and Bidobacterium dentium with respect to the control group. Moreover, this study has preliminarily evaluated that some bifidobacterial strains, previously selected for use in infants, have antibacterial properties against GBS and are therefore potential candidates for being applied as probiotics for the prevention of GBS infections.

Keywords

Intrapartum antibiotic prophylaxis Group B Streptococcus Bifidobacterium spp Probiotics Antibacterial activity 

References

  1. Aloisio I, Santini C, Biavati B, Dinelli G, Cencič A, Chingwaru W, Mogna L, Di Gioia D (2012) Characterization of Bifidobacterium spp. strains for the treatment of enteric disorders in newborns. Appl Microbiol Biotechnol 96:1561–1576PubMedCrossRefGoogle Scholar
  2. Al-Taiar A, Hammoud M, Thalib L, Isaacs D (2011) Pattern and etiology of culture-proven early onset neonatal sepsis: a five year prospective study. Int J Infec Dis 15:631–634CrossRefGoogle Scholar
  3. Altschul S, Gish W, Miller W, Myers E, Lipman D (1990) Basic local alignment search tool. J Mol Biol 215:403–410PubMedCrossRefGoogle Scholar
  4. Amaretti A, di Nunzio M, Pompei A, Raimondi S, Rossi M, Bordoni A (2013) Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities. Appl Microbiol Biotechnol 97:809–817PubMedCrossRefGoogle Scholar
  5. Ammor MS, Flórez AB, van Hoek AHAM, de Los Reyes-Gavilán CG, Aarts HJM, Margolles A, Mayo B (2008) Molecular characterization of intrinsic and acquired antibiotic resistance in lactic acid bacteria and bifidobacteria. J Mol Microbiol Biotechnol 14:6–15PubMedCrossRefGoogle Scholar
  6. Bäckhed F, Fraser CM, Ringel Y, Sanders ME, Sartor RB, Sherman PM, Versalovic J, Young V, Finlay BB (2012) Defining a healthy human gut microbiome: current concepts, future directions, and clinical applications. Cell Host Microbe 12:611–622PubMedCrossRefGoogle Scholar
  7. Benzecri J (1992) Correspondance analyses handbook. CRC Press, Boca Raton, FloridaGoogle Scholar
  8. Bizzarro M, Dembry L, Baltimore R, Gallagher P (2008) Changing patterns in neonatal Escherichia coli sepsis and ampicillin resistance in the era of intrapartum antibiotic prophylaxis. Pediatrics 121:689–696PubMedCrossRefGoogle Scholar
  9. Bush K, Courvalin P, Dantas G, Davies J, Eisenstein B, Huovinen P, Jacoby GA, Kishony R, Kreiswirth BN, Kutter E, Lerner SA, Levy S, Lewis K, Lomovskaya O, Miller JH, Mobashery S, Piddock LJV, Projan S, Thomas CM, Tomasz A, Tulkens PM, Walsh TR, Watson JD, Witkowski J, Witte W, Wright G, Yeh P, Zgurskaya HI (2011) Tackling antibiotic resistance. Nat Rev Microbiol 9:894–896PubMedCrossRefGoogle Scholar
  10. Cani PD, Delzenne NM (2011) The gut microbiome as therapeutic target. Pharmacol Ther 130:202–212PubMedCrossRefGoogle Scholar
  11. Castillo M, Martín-Orúe SM, Manzanilla EG, Badiola I, Martín M, Gasa J (2006) Quantification of total bacteria, enterobacteria and lactobacilli populations in pig digesta by real-time PCR. Vet Microbiol 114:165–170PubMedCrossRefGoogle Scholar
  12. Chiang S-S, Pan T-M (2012) Beneficial effects of Lactobacillus paracasei subsp. paracasei NTU 101 and its fermented products. Appl Microbiol Biotechnol 93:903–916PubMedCrossRefGoogle Scholar
  13. D’Aimmo MR, Modesto M, Biavati B (2007) Antibiotic resistance of lactic acid bacteria and Bifidobacterium spp. isolated from dairy and pharmaceutical products. Int J Food Microbiol 115:35–42PubMedCrossRefGoogle Scholar
  14. Di Gioia D, Aloisio I, Mazzola G, Biavati B (2014) Bifidobacteria: their impact on gut microbiota composition and their applications as probiotics in infants. Appl Microbiol Biotechnol 98:563–577PubMedCrossRefGoogle Scholar
  15. Dotterud CK, Storrø O, Johnsen R, Oien T (2010) Probiotics in pregnant women to prevent allergic disease: a randomized, double-blind trial. Br J Dermatol 163:616–623PubMedCrossRefGoogle Scholar
  16. Ferrieri P, Wallen L (2012) Neonatal bacterial sepsis. In: Gleason CA, Devaskar S (eds) Avery’s disease of the newborn, 9th edn. Elsevier Saunders, Philadelphia, p 538–550Google Scholar
  17. Fuligni F, Gueimende M, Margolles A, De Bellis G, van O’Toole PW, Sinderen D, Marchesi JR, Ventura M (2012) Diversity of bifidobacteria within the infant gut microbiota. PLoS ONE 7:e36957PubMedCentralPubMedCrossRefGoogle Scholar
  18. Grice EA, Segre JA (2012) The human microbiome: our second genome. Annu Rev Genomics Hum Genet 13:151–170PubMedCentralPubMedCrossRefGoogle Scholar
  19. Keski-Nisula L, Kyynäräinen H-R, Kärkkäinen U, Karhukorpi J, Heinonen S, Pekkanen J (2013) Maternal intrapartum antibiotics and decreased vertical transmission of Lactobacillus to neonates during birth. Acta Paediatr 102:480–485PubMedCrossRefGoogle Scholar
  20. Klappenbach J, Saxman P, Cole J, Schmidt T (2001) rrnDB: the ribosomal RNA operon copy number database. Nucleic Acids Res 29:181–184PubMedCentralPubMedCrossRefGoogle Scholar
  21. Lee Z, Bussema C, Schmidt T (2009) rrnDB: documenting the number of rRNA and tRNA genes in bacteria and archaea. Nucleic Acids Res 37:489–493CrossRefGoogle Scholar
  22. Malinen E, Kassinen A, Rinttila T, Palva A (2003) Comparison of real-time PCR with SYBR Green I or 5′-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria. Microbiology 149:269–277PubMedCrossRefGoogle Scholar
  23. Mangin I, Suau A, Gotteland M, Brunser O, Pochart P (2010) Amoxicillin treatment modifies the composition of Bifidobacterium species in infant intestinal microbiota. Anaerobe 16:433–438PubMedCrossRefGoogle Scholar
  24. Mättö J, van Hoek AHAM, Domig KJ, Saarela M, Floréz AB, Brockmann E, Amtmann E, Mayo B, Aarts HJM, Danielsen M (2007) Susceptibility of human and probiotic Bifidobacterium spp. to selected antibiotics as determined by the Etest method. Int Dairy J 17:1123–1131CrossRefGoogle Scholar
  25. Muyzer G, Smalla K (1998) Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Anton Leeuw 73:127–141CrossRefGoogle Scholar
  26. O’Sullivan A, He X, McNiven EMS, Haggarty NW, Lönnerdal B, Slupsky CM (2013) Early diet impacts infant rhesus gut microbiome, immunity, and metabolism. J Proteome Res 12:2833–2845PubMedCrossRefGoogle Scholar
  27. Palmer C, Bik E, DiGiulio D, Relman D, Brown P (2007) Development of the human infant intestinal microbiota. PLoS Biol 5:1556–1573CrossRefGoogle Scholar
  28. Penders J, Thijs C, Vink C, Stelma F, Snijders B, Kummeling I, van den Brandt P (2006) Factors influencing the composition of the intestinal microbiota in early infancy. Pediatrics 118:511–521PubMedCrossRefGoogle Scholar
  29. Phillips EA, Tapsall JW, Smith DD (1980) Rapid tube CAMP test for identification of Streptococcus agalactiae (Lancefield group B). J Clin Microbiol 12:135–137PubMedCentralPubMedGoogle Scholar
  30. Puopolo K, Madoff L, Eichenwald E (2005) Early-onset group B streptococcal disease in the era of maternal screening. Pediatrics 115:1240–1246PubMedCrossRefGoogle Scholar
  31. Rinttilä T, Kassinen A, Malinen E, Krogius L, Palva A (2004) Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol 97:1166–1177PubMedCrossRefGoogle Scholar
  32. Sanders M, Akkermans L, Haller D, Hammerman C, Heimbach J, Hörmannsperger G, Huys G (2010) Safety assessment of probiotics for human use. Gut Microbes 1:164–185PubMedCentralPubMedCrossRefGoogle Scholar
  33. Santini C, Baffoni L, Gaggia F, Granata M, Gasbarri R, Di Gioia D, Biavati B (2010) Characterization of probiotic strains: an application as feed additives in poultry against Campylobacter jejuni. Int J Food Microbiol 141:S98–S108PubMedCrossRefGoogle Scholar
  34. Sanz Y, Sánchez E, Marzotto M, Calabuig M, Torriani S, Dellaglio F (2007) Differences in faecal bacterial communities in celiac and healthy children as detected by PCR and denaturing gradient gel electrophoresis. FEMS Immunol Med Microbiol 51:562–568PubMedCrossRefGoogle Scholar
  35. Satokari RM, Vaughan EE, Akkermans ADL, Saarela M (2001) Bifidobacterial diversity in human feces detected by genus- specific pcr and denaturing gradient gel electrophoresis. Appl Environ Microbiol 67:504–513PubMedCentralPubMedCrossRefGoogle Scholar
  36. Satokari R, Vaughan E, Favier F, Dore J, Edwards C, de Vos W (2002) Diversity of Bifidobacterium and Lactobacillus spp. in breast-fed and formula-fed infants as assessed by 16S rDNA sequence differences. Microb Ecol Health Dis 14:97–105CrossRefGoogle Scholar
  37. Savino F, Cordisco L, Tarasco V, Locatelli E, Di Gioia D, Oggero R (2011) Antagonistic effect of Lactobacillus strains against gas-producing coliforms isolated from colicky infants. BMC Microb 11:157CrossRefGoogle Scholar
  38. Schloissnig S, Arumugam M, Sunagawa S, Mitreva M, Tap J, Zhu A, Waller A, Mende DR, Kultima JR, Martin J, Kota K, Sunyaev SR, Weinstock GM, Bork P (2013) Genomic variation landscape of the human gut microbiome. Nature 493:45–50PubMedCentralPubMedCrossRefGoogle Scholar
  39. Sharon I, Morowitz MJ, Thomas BC, Costello EK, Relman DA, Banfield JF (2013) Time series community genomics analysis reveals rapid shifts in bacterial species, strains, and phage during infant gut colonization. Genome Res 23:111–120PubMedCentralPubMedCrossRefGoogle Scholar
  40. Solís G, de Los R-GC, Fernández N, Margolles A, Gueimonde M (2010) Establishment and development of lactic acid bacteria and bifidobacteria microbiota in breast-milk and the infant gut. Anaerobe 16:307–310PubMedCrossRefGoogle Scholar
  41. Turroni F, Peano C, Pass DA, Foroni E, Severgnini M, Claesson MJ, Kerr C, Hourihane J, Murray D, Fuligni F, Gueimonde M, Margolles A, De Bellis G, O’Toole PW, van Sinderen D, Marchesi JR, Ventura M (2012) Diversity of bifidobacteria within the infant gut microbiota. PLoS One 7:e36957PubMedCentralPubMedCrossRefGoogle Scholar
  42. Wickens K, Black PN, Stanley TV, Mitchell E, Fitzharris P, Tannock GW, Purdie G, Crane J (2008) A differential effect of 2 probiotics in the prevention of eczema and atopy: a double-blind, randomized, placebo-controlled trial. J Allergy Clin Immunol 122:788–794PubMedCrossRefGoogle Scholar
  43. Zárate G, Nader-Macias ME (2006) Influence of probiotic vaginal lactobacilli on in vitro adhesion of urogenital pathogens to vaginal epithelial cells. Lett Appl Microbiol 43:174–180PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Irene Aloisio
    • 1
  • Giuseppe Mazzola
    • 1
  • Luigi Tommaso Corvaglia
    • 2
  • Giacomo Tonti
    • 2
  • Giacomo Faldella
    • 2
  • Bruno Biavati
    • 1
  • Diana Di Gioia
    • 1
    Email author
  1. 1.Department of Agricultural SciencesUniversity of BolognaBolognaItaly
  2. 2.Neonatal Intensive Care UnitS. Orsola Malpighi HospitalBolognaItaly

Personalised recommendations