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Modulation of peanut-induced allergic immune responses by oral lactic acid bacteria-based vaccines in mice

  • Applied genetics and molecular biotechnology
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Abstract

Peanut allergy (PNA) has becoming a non-negligible health concern worldwide. Thus far, allergen-specific immunotherapy aimed at inducing mucosal tolerance has widely been regarded as a major management strategy for PNA. The safety profiles and the intrinsic probiotic properties of lactic acid bacteria (LAB) render them attractive delivery vehicles for mucosal vaccines. In the present study, we exploited genetically modified Lactococcus lactis to produce peanut allergen Ara h 2 via different protein-targeting systems and their immunomodulatory potency for allergic immune responses in mice were investigated. By comparison with the strain expressing the cytoplasmic form of Ara h 2 (LL1), the strains expressing the secreted and anchored forms of Ara h 2 (LL2 and LL3) were more potent in redirecting a Th2-polarized to a non-allergic Th1 immune responses. Induction of SIgA and regulatory T cells were also observed at the local levels by orally administration of recombinant L. lactis. Our results indicate that allergen-producing L. lactis strains modulated allergic immune responses and may be developed as promising mucosal vaccines for managing allergic diseases.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31200691), the National Science Fund for Distinguished Young Scholars (No. 31125021), the National Science & Technology Pillar Program in the Twelfth Five-year Plan Period (No. 2013BAD18B01, 2013BAD18B02, 2012BAD28B07, 2012BAD28B08), the 111 project B07029, IRT1249, and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Chengcheng Ren, Qiuxiang Zhang, Gang Wang, Chunqing Ai, Mengsha Hu, Xiaoming Liu, Fengwei Tian, Jianxin Zhao, Yongquan Chen, Miao Wang, Hao Zhang & Wei Chen

  2. Synergistic Innovation Center for Food Safety and Nutrition, Wuxi, 214122, China

    Yongquan Chen & Wei Chen

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  1. Chengcheng Ren
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  2. Qiuxiang Zhang
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  3. Gang Wang
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  11. Hao Zhang
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Correspondence to Hao Zhang or Wei Chen.

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Ren, C., Zhang, Q., Wang, G. et al. Modulation of peanut-induced allergic immune responses by oral lactic acid bacteria-based vaccines in mice. Appl Microbiol Biotechnol 98, 6353–6364 (2014). https://doi.org/10.1007/s00253-014-5678-7

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