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Genetic surgery in fungi: employing site-specific recombinases for genome manipulation

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Abstract

Site-specific recombination mediates the rearrangement of nucleic acids by the virtue of an recombinase acting on specific recognition sequences. Recombining activities belong either to the tyrosine- or serine-type group, based on the presence of specific residues in the catalytic centre, which can be further subdivided into families due to additional criteria. The most prominent systems are the λ phage integrase acting on att sites; the Cre recombinase from bacteriophage P1 with its loxP attachment sites; the FLP/FTR system of fungal origin, where it is required for 2-μm plasmid replication/amplification in yeast; and the prokaryotic β-recombinase that recombines six sites specifically in cis. Each of these has been exploited in fungal hosts of biotechnological, medical or general relevance, mainly for cloning projects, approaches of gene targeting, genome modification or screening purposes. With their precise and defined mode of action are site-specific recombination systems eminently suited for genetic tasks in fungi, like they are executed in functional studies at high throughput or modern approaches of synthetic biology.

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Acknowledgments

The author's research is funded by the German Research Foundation (KR 2294/3-1), the Microbiology Institute at the University Hospital of Erlangen and the University of Erlangen-Nürnberg (ELAN project MH-12-08-17-1); further support had been granted by the European Science Foundation via the FUMINOMICS Research Networking Programme (06-RNP-132). Prof. Dr. Christian Bogdan is thanked for critically reading the manuscript, as is all other members of the Microbiology Institute for support and assistance. I apologise to those whose work was not cited for the sake of conciseness. This review is dedicated to Livia Pauline for recombining my everyday life on a daily basis.

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  1. Mikrobiologisches Institut - Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Wasserturmstr. 3/5, 91054, Erlangen, Germany

    Sven Krappmann

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  1. Sven Krappmann
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Krappmann, S. Genetic surgery in fungi: employing site-specific recombinases for genome manipulation. Appl Microbiol Biotechnol 98, 1971–1982 (2014). https://doi.org/10.1007/s00253-013-5480-y

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