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Toxic effects of nanoparticles on bioluminescence activity, seed germination, and gene mutation

  • Environmental biotechnology
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Abstract

The potential environmental toxicities of several metal oxide nanoparticles (NPs; CuO, TiO2, NiO, Fe2O3, ZnO, and Co3O4) were evaluated in the context of bioluminescence activity, seed germination, and bacterial gene mutation. The bioassays exhibited different sensitivities, i.e., each kind of NP exhibited a different level of toxicity in each of the bioassays. However, with a few exceptions, CuO and ZnO NPs had most toxic for germination of Lactuca seed (EC50 0.46 mg CuO/l) and bioluminescence (EC50 1.05 mg ZnO/l). Three NPs (Co3O4, TiO2, and Fe2O3) among all tested concentrations (max. 1,000 mg/l) showed no inhibitory effects on the tested organisms, except for Co3O4 NPs on bioluminescence activity (EC50 62.04 mg/l). The sensitivity of Lactuca seeds was greater than that of Raphanus seeds (EC50 0.46 mg CuO/l versus 26.84 mg CuO /l ). The ranking of metal toxicity levels on bioluminescence was in the order of ZnO > CuO > Co3O4 > NiO > Fe2O3, TiO2, while CuO > ZnO > NiO > Co3O4, Fe2O3, TiO2 on germination. No revertant mutagenic ratio (greater than 2.0) of Salmonella typhimurium TA 98 was observed under any tested condition. These findings demonstrate that several bioassays, as opposed to any single one, are needed for the accurate assessment of NP toxicity on ecosystems.

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Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0022750). Also this research was partly supported by the Basic Research Project of the Korea Institute of Geoscience and Mineral Resources (KIGAM) funded by the Ministry of Knowledge and Economy. We also would like to thank Prof. Yinjie Tang (Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, MO, USA) for his efforts in helping project design.

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Authors and Affiliations

  1. Geologic Environment Division, Korea Institute of Geoscience and Mineral Resources (KIGAM), Daejeon, South Korea

    Kyung-Seok Ko

  2. Department of Environmental Engineering, Yeungnam University, Gyungsan-si, Kyungbuk, 712-749, South Korea

    In Chul Kong

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  1. Kyung-Seok Ko
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  2. In Chul Kong
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Correspondence to In Chul Kong.

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Ko, KS., Kong, I.C. Toxic effects of nanoparticles on bioluminescence activity, seed germination, and gene mutation. Appl Microbiol Biotechnol 98, 3295–3303 (2014). https://doi.org/10.1007/s00253-013-5404-x

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  • DOI: https://doi.org/10.1007/s00253-013-5404-x

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