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Antrodia camphorata-fermented product cultured in deep ocean water has more liver protection against thioacetamide-induced fibrosis

Abstract

Antrodia camphorata is a unique fungus in Taiwan; the submerged fermentation product is used as the functional food for liver protection. Deep ocean water (DOW) containing rich metals and trace elements is proven to stimulate the production of functional metabolites and health function of functional fungus product in our previous study. Therefore, A. camphorata-fermented product cultured in DOW (DOW-AC) or reverse osmosis water (ROW-AC) as culture water was daily fed thioacetamide (TAA)-induced fibrosis rat for 8 weeks in order to investigate whether DOW promoted the effect of A. camphorata-fermented product on the prevention against TAA-induced liver damage and fibrosis. In the results, feeding one dose of DOW-AC prevented from TAA-induced weight loss and had more effect on inhibiting lipid peroxidation, reactive oxygen species, iNOS, and TNF-α expression than one dose of ROW-AC. Furthermore, DOW-AC also had more potent effect on protection against TAA-induced liver damage and fibrosis according to the results of H&E stain and collagen stain. However, higher liver protection of DOW-AC should be due to the fact that DOW not only increased the production of A. camphorata-fermented functional metabolites including triterpenoids, polysaccharides, flavonoids, and polyphenols but also contributed to protection against TAA-induced damage and fibrosis.

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Acknowledgments

This study was supported by a grant from the National Science Council, Republic of China (NSC-98-2313-B-143-002-MY3), Industrial Technology Research Institute of Taiwan, Republic of China (B200-101-YG-02), and Ministry of Economic Affairs, Republic of China (102-EC-17-A-32-S1-230).

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Correspondence to Chun-Lin Lee.

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Wang, LC., Kuo, IU., Tsai, TY. et al. Antrodia camphorata-fermented product cultured in deep ocean water has more liver protection against thioacetamide-induced fibrosis. Appl Microbiol Biotechnol 97, 9955–9967 (2013). https://doi.org/10.1007/s00253-013-5214-1

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  • DOI: https://doi.org/10.1007/s00253-013-5214-1

Keywords

  • Antrodia camphorata
  • Deep ocean water
  • Liver fibrosis
  • Thioacetamide