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Microbial community structure of a pilot-scale thermophilic anaerobic digester treating poultry litter

Applied Microbiology and Biotechnology volume 98, pages 2321–2334 (2014)Cite this article

Abstract

The microbial community structure of a stable pilot-scale thermophilic continuous stirred tank reactor digester stabilized on poultry litter was investigated. This 40-m3 digester produced biogas with 57 % methane, and chemical oxygen demand removal of 54 %. Bacterial and archaeal diversity were examined using both cloning and pyrosequencing that targeted 16S rRNA genes. The bacterial community was dominated by phylum Firmicutes, constituting 93 % of the clones and 76 % of the pyrotags. Of the Firmicutes, class Clostridia (52 % pyrotags) was most abundant followed by class Bacilli (13 % pyrotags). The bacterial libraries identified 94 operational taxonomic units (OTUs) and pyrosequencing identified 577 OTUs at the 97 % minimum similarity level. Fifteen OTUs were dominant (≥2 % abundance), and nine of these were novel unclassified Firmicutes. Several of the dominant OTUs could not be classified more specifically than Clostridiales, but were most similar to plant biomass degraders, including Clostridium thermocellum. Of the rare pyrotag OTUs (<0.5 % abundance), 75 % were Firmicutes. The dominant methanogen was Methanothermobacter which has hydrogenotrophic metabolism, and accounted for >99 % of the archaeal clones. Based on the primary methanogen, as well as digester chemistry (high VA and ammonia levels), we propose that bacterial acetate oxidation is the primary pathway in this digester for the control of acetate levels.

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Acknowledgements

This research was funded by USDA CSREES grant 2004–02614. We also thank the Gus R. Douglass Land Grant Institute (WVSU) for support, John Bombardiere and Jesus E. Chavarria Palma for assistance, and Nagamani Balagurusamy for helpful comments.

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  1. Department of Biology, West Virginia State University, Hamblin Hall, Institute, WV, 25112-1000, USA

    Ami M. Smith, Deepak Sharma & David H. Huber

  2. Gus R. Douglass Institute, West Virginia State University, Institute, WV, 25112, USA

    Deepak Sharma & David H. Huber

  3. Department of Biosciences, Swansea University, Wallace Building, Singleton Park, Swansea, SA2 8PP, Wales, UK

    Hilary Lappin-Scott

  4. Biosciences, College of Life and Environmental Sciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK

    Ami M. Smith & Sara Burton

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Smith, A.M., Sharma, D., Lappin-Scott, H. et al. Microbial community structure of a pilot-scale thermophilic anaerobic digester treating poultry litter. Appl Microbiol Biotechnol 98, 2321–2334 (2014). https://doi.org/10.1007/s00253-013-5144-y

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