Applied Microbiology and Biotechnology

, Volume 98, Issue 3, pp 1195–1203

Penicillin G acylase from Achromobacter sp. CCM 4824

An efficient biocatalyst for syntheses of beta-lactam antibiotics under conditions employed in large-scale processes
  • Stanislav Bečka
  • Václav Štěpánek
  • Rajasekar W. Vyasarayani
  • Michal Grulich
  • Jaroslav Maršálek
  • Kamila Plháčková
  • Marie Dobišová
  • Helena Marešová
  • Martina Plačková
  • Renáta Valešová
  • Andrea Palyzová
  • Anupama Datla
  • Trupti K. Ashar
  • Pavel Kyslík
Biotechnologically relevant enzymes and proteins

DOI: 10.1007/s00253-013-4945-3

Cite this article as:
Bečka, S., Štěpánek, V., Vyasarayani, R.W. et al. Appl Microbiol Biotechnol (2014) 98: 1195. doi:10.1007/s00253-013-4945-3

Abstract

Penicillin G acylase from Achromobacter sp. (NPGA) was studied in the enzymatic synthesis of β-lactam antibiotics by kinetically controlled N-acylation. When compared with penicillin acylase of Escherichia coli (PGA), the NPGA was significantly more efficient at syntheses of ampicillin and amoxicillin (higher S/H ratio and product accumulation) in the whole range of substrate concentrations. The degree of conversion of 6-aminopenicillanic acid to amoxicillin and ampicillin (160 mM 6-APA, 350 mM acyl donor methylester⋅HCl, pH 6.3, 25 °C, reaction time of 200 min) with immobilized NPGA equaled 96.9 % and 91.1 %, respectively. The enzyme was highly thermostable with maximum activity at 60 °C (pH 8.0) and 65 °C (pH 6.0). Activity half-life at 60 °C (pH 8.0) and at 60 °C (pH 6.0) was 24 min and 6.9 h, respectively. Immobilized NPGA exhibited long operational stability with half-life of about 2,000 cycles for synthesis of amoxicillin at conversion conditions used in large-scale processes (230 mM 6-APA, 340 mM d-4-hydroxyphenylglycine methylester⋅HCl, 27.5 °C, pH 6.25). We discuss our results with literature data available for related penicillin acylases in terms of their industrial potential.

Keywords

Achromobacter sp. Penicillin G acylase β-Lactam antibiotics Kinetically controlled synthesis Immobilized enzyme 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Stanislav Bečka
    • 1
  • Václav Štěpánek
    • 1
  • Rajasekar W. Vyasarayani
    • 2
  • Michal Grulich
    • 1
  • Jaroslav Maršálek
    • 1
  • Kamila Plháčková
    • 1
  • Marie Dobišová
    • 1
  • Helena Marešová
    • 1
  • Martina Plačková
    • 1
  • Renáta Valešová
    • 1
  • Andrea Palyzová
    • 1
  • Anupama Datla
    • 2
  • Trupti K. Ashar
    • 2
  • Pavel Kyslík
    • 1
  1. 1.Laboratory of Enzyme TechnologyInstitute of Microbiology ASCR, v.v.i.PragueCzech Republic
  2. 2.Fermenta Biotech Ltd.ThaneIndia

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