Applied Microbiology and Biotechnology

, Volume 97, Issue 7, pp 2761–2772 | Cite as

Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation

  • Won-Heong Lee
  • Myoung-Dong Kim
  • Yong-Su Jin
  • Jin-Ho Seo


Efficient regeneration of NADPH is one of the limiting factors that constrain the productivity of biotransformation processes. In order to increase the availability of NADPH for enhanced biotransformation by engineered Escherichia coli, modulation of the pentose phosphate pathway and amplification of the transhydrogenases system have been conventionally attempted as primary solutions. Recently, other approaches for stimulating NADPH regeneration during glycolysis, such as replacement of native glyceradehdye-3-phosphate dehydrogenase (GAPDH) with NADP-dependent GAPDH from Clostridium acetobutylicum and introduction of NADH kinase catalyzing direct phosphorylation of NADH to NADPH from Saccharomyces cerevisiae, were attempted and resulted in remarkable impacts on NADPH-dependent bioprocesses. This review summarizes several metabolic engineering approaches used for improving the NADPH regenerating capacity in engineered E. coli for whole-cell-based bioprocesses and discusses the key features and progress of those attempts.


Biotransformation process Engineered Escherichia coli NADPH regeneration Pentose phosphate pathway Transhydrogenase NADP-dependent glyceradehdye-3-phosphate dehydrogenase NAD(H) kinase 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Won-Heong Lee
    • 1
    • 2
  • Myoung-Dong Kim
    • 3
  • Yong-Su Jin
    • 2
  • Jin-Ho Seo
    • 1
  1. 1.Department of Agricultural Biotechnology and Center for Food and BioconvergenceSeoul National UniversitySeoulKorea
  2. 2.Department of Food Science and Human Nutrition and Institute for Genomic BiologyUniversity of Illinois at Urbana-ChampaignUrbanaUSA
  3. 3.Department of Food Science and BiotechnologyKangwon National UniversityChuncheonKorea

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