Applied Microbiology and Biotechnology

, Volume 97, Issue 12, pp 5457–5471 | Cite as

Infection of tomato leaf curl New Delhi virus (ToLCNDV), a bipartite begomovirus with betasatellites, results in enhanced level of helper virus components and antagonistic interaction between DNA B and betasatellites

  • P. Jyothsna
  • Q. M. I. Haq
  • Priyanka Singh
  • K. V. Sumiya
  • Shelly Praveen
  • Ramaveer Rawat
  • Rob W. Briddon
  • V. G. MalathiEmail author
Applied genetics and molecular biotechnology


Tomato leaf curl New Delhi virus (ToLCNDV) (Geminiviridae) is an important pathogen that severely affects tomato production. An extensive survey was carried out during 2003–2010 to study the diversity of begomoviruses found in tomato, potato, and cucurbits that showed symptoms of leaf puckering, distortion, curling, vein clearing, and yellow mosaic in various fields in different regions of India. Ten begomovirus isolates were cloned from infected samples and identified as belonging to the species ToLCNDV. A total of 44 % of the samples showed association of betasatellites, with CLCuMuB and LuLDB being the most frequent. The ToLCNDV cloned component DNA A and DNA B were agroinoculated on Nicotiana benthamiana and tomato (Solanum lycopersicum) plants with or without betasatellites, CLCuMuB or LuLDB. The viral genome levels were then monitored by real-time polymerase chain reaction at different time points of disease development. Plants co-inoculated with betasatellites showed enhanced symptom severity in both N. benthamiana and tomato, as well as increases in helper viral DNA A and DNA B levels. The DNA B and betasatellites acted antagonistically to each other, so that the level of DNA B was 16-fold greater in the presence of betasatellites, while accumulation of betasatellites, CLCuMuB and LuLDB, were reduced by 60 % in the presence of DNA B. DNA B-mediated symptoms predominated in CLCuMuB-inoculated plants, whereas betasatellite-mediated leaf abnormalities were prominent in LuLDB-co-inoculated plants. Inoculation with the cloned components will be a good biotechnological tool in resistance breeding program.


Begomovirus Tomato leaf curl New Delhi virus Antagonism DNA B Betasatellites 



The authors are grateful for the facilities provided by Dr. H.S. Gupta, The Director, Indian Agricultural Research Institute and Dr. R.K. Jain, Head, Division of Plant Pathology in conducting this study. The authors are also thankful to Darren P. Martin and Brejnev Muhire, Institute of Infectious Disease and Molecular Medicine, Computational Biology Group, University of Cape Town, South Africa for their guidance in the SDT analysis. The financial assistance by the Department of Biotechnology, Government of India, Project BT/PR/5351/AGR/16/482/2004-II is duly acknowledged. The support of the National Phytotron Facility is highly appreciated.

Supplementary material

253_2012_4685_MOESM1_ESM.pdf (379 kb)
ESM 1 (PDF 378 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • P. Jyothsna
    • 1
  • Q. M. I. Haq
    • 1
  • Priyanka Singh
    • 1
  • K. V. Sumiya
    • 1
  • Shelly Praveen
    • 1
  • Ramaveer Rawat
    • 2
  • Rob W. Briddon
    • 3
  • V. G. Malathi
    • 1
    Email author
  1. 1.Advanced Centre for Plant Virology, Division of Plant PathologyIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.MM (PG) CollegeModinagarIndia
  3. 3.National Institute for Biotechnology and Genetic EngineeringFaisalabadPakistan

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