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Evaluation of anti-quorum sensing activity of silver nanowires

Applied Microbiology and Biotechnology volume 97, pages 3593–3601 (2013)Cite this article

Abstract

A menace of antimicrobial resistance with growing difficulties in eradicating clinical pathogens owing to the biofilm has prompted us to take up a facile aqueous-phase approach for the synthesis of silver nanowires (SNWs) by using ethylene glycol as a reducing agent and polyvinylpyrrolidone (PVP) as a capping agent. This synthesis is a reflux reaction seedless process. The obtained SNWs were about 200–250 nm in diameter and up to 3–4 μm in length. The SNWs were characterized by field emission scanning electron microscopy, transmission electron microscopy, UV–Vis spectroscopy, and X-Ray powder diffraction, and the chemical composition of the sample was examined by energy dispersive X-ray spectrum. The SNWs did not show an antibacterial activity against test organisms, Bacillus subtilis NCIM 2063 and Escherichia coli NCIM 2931; however, it showed a promising property of a quorum sensing-mediated inhibition of biofilm in Pseudomonas aeruginosa NCIM 2948 and violacein synthesis in Chromobacterium violaceum ATCC 12472, which is hitherto unattempted, by polyol approach.

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Acknowledgments

The authors would like to thank Dr. D. P. Amalnerkar, Executive Director, C-MET, for constant encouragement throughout this work and the Department of Electronics and Information Technology (DeitY), New Delhi for the financial support. RP would like to thank UGC-SRF, and DT would like to thank DST-PURSE programme for the financial assistance.

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Affiliations

  1. Nanocomposite/Glass Laboratory, Centre for Materials for Electronics Technology (C-MET), Department of Information Technology, Government of India, Panchawati, Off Pashan Road, Pune, 411008, India

    Mohini S. Wagh (nee Jagtap), Milind V. Kulkarni & Bharat B. Kale

  2. Department of Biotechnology, University of Pune, Pune, 411007, India

    Rajendra H. Patil, Deepali K. Thombre & Wasudev N. Gade

Authors
  1. Mohini S. Wagh (nee Jagtap)
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  2. Rajendra H. Patil
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  3. Deepali K. Thombre
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  4. Milind V. Kulkarni
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  5. Wasudev N. Gade
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  6. Bharat B. Kale
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Corresponding author

Correspondence to Milind V. Kulkarni.

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Wagh (nee Jagtap), M.S., Patil, R.H., Thombre, D.K. et al. Evaluation of anti-quorum sensing activity of silver nanowires. Appl Microbiol Biotechnol 97, 3593–3601 (2013). https://doi.org/10.1007/s00253-012-4603-1

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  • DOI: https://doi.org/10.1007/s00253-012-4603-1

Keywords

  • Silver nanowires
  • Polyol
  • Anti-quorum
  • Pseudomonas
  • CLSM