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Applied Microbiology and Biotechnology

, Volume 97, Issue 19, pp 8795–8804 | Cite as

Microbial activity of suspended biomass from a nitritation–anammox SBR in dependence of operational condition and size fraction

  • Eva Marianne GilbertEmail author
  • Elisabeth Müller
  • Harald Horn
  • Susanne Lackner
Environmental biotechnology

Abstract

Single-stage nitritation–anammox combines the growth of aerobic ammonium-oxidizing bacteria (AOB) and anaerobic ammonium oxidizing bacteria (AnAOB) in one reactor. The necessary compromise of their milieu conditions often leads to the growth of nitrite-oxidizing bacteria (NOB). For this study, a sequencing batch reactor (SBR) for nitritation–anammox was operated for 180 days with sewage sludge reject water (removal capacity, 0.4 kg N m−3 day−1). The growth of NOB was favored by enhanced oxygen supply rather than extended aerobic phases. Suspended-type biomass from this SBR was taken regularly and sieved into three size fractions (all of them <1,000 μm). Batch experiments as well as fluorescence in situ hybridization were performed to study the distribution and activity of AnAOB, AOB, and NOB within those size fractions. Both the measured conversion rates and detected abundances decreased with increasing size fraction. The highest anammox conversion rates (15 g NH4 +–N per kilogram VSS per hour) and the highest abundances of Brocadia fulgida were found in the medium size fraction (100–315 μm). The batch experiments proved to be accurate tools for the monitoring of multiple processes in the reactor. The results were representative for reactor performance during the 6 months of reactor operation.

Keywords

Nitritation–anammox Nitratation Suspended biomass Particle size distribution Microbial distribution Batch experiments Activity measurements 

Notes

Acknowledgment

This research was funded by the Bavarian Environment Agency. The authors would like to thank Susanne Thiemann (Institute of Water Quality Control, Technical University Munich) for FISH analyses and Steffen Krause (Sanitary Engineering and Waste Management, Bundeswehr Universty Munich) for supply and assistance with the particle size analyses.

Supplementary material

253_2012_4591_MOESM1_ESM.docx (315 kb)
ESM 1 (DOCX 315 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Eva Marianne Gilbert
    • 1
    Email author
  • Elisabeth Müller
    • 2
  • Harald Horn
    • 1
  • Susanne Lackner
    • 1
  1. 1.Engler-Bunte-Institute, Water Chemistry and Water TechnologyKarlsruhe Institute of TechnologyKarlsruheGermany
  2. 2.Institute of Water and Environment, Water Quality ControlTechnische Universität MünchenGarchingGermany

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