Applied Microbiology and Biotechnology

, Volume 97, Issue 19, pp 8813–8821 | Cite as

Perhydrolase-nanotube paint composites with sporicidal and antiviral activity

  • Navdeep Grover
  • Marc P. Douaisi
  • Indrakant V. Borkar
  • Lillian Lee
  • Cerasela Zoica Dinu
  • Ravi S. Kane
  • Jonathan S. Dordick
Environmental biotechnology


AcT (perhydrolase) containing paint composites were prepared leading to broad-spectrum decontamination. AcT was immobilized onto multi-walled carbon nanotubes (MWNTs) and then incorporated into latex-based paints to form catalytic coatings. These AcT-based paint composites showed a 6-log reduction in the viability of spores of Bacillus cereus and Bacillus anthracis (Sterne) within 60 min. The paint composites also showed >4-log reduction in the titer of influenza virus (X-31) within 10 min (initially challenged with 107 PFU/mL). AcT-based paint composites were also tested using various perhydrolase acyl donor substrates, including propylene glycol diacetate (PGD), glyceryl triacetate, and ethyl acetate, with PGD observed to be the best among the substrates tested for generation of peracetic acid and killing of bacillus spores. The operational stability of paint composites was also studied at different relative humidities and temperatures to simulate real-life operation.


Perhydrolase AcT B. cereus and B. anthracis spores Influenza virus Multi-walled carbon nanotubes Nanocomposites 



This work was supported by the U.S. Army Corps of Engineers under contracts W913T-10-2-0006 and W913T-11-R-0033.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Navdeep Grover
    • 1
  • Marc P. Douaisi
    • 1
  • Indrakant V. Borkar
    • 1
  • Lillian Lee
    • 1
  • Cerasela Zoica Dinu
    • 2
  • Ravi S. Kane
    • 1
    • 3
  • Jonathan S. Dordick
    • 1
    • 4
  1. 1.Department of Chemical and Biological Engineering, Rensselaer Nanotechnology Center, and Center for Biotechnology & Interdisciplinary StudiesRensselaer Polytechnic InstituteTroyUSA
  2. 2.Department of Chemical Engineering, Benjamin M. Statler College of Engineering and Mineral ResourcesWest Virginia UniversityMorgantownUSA
  3. 3.Department of Chemical and Biological EngineeringRensselaer Polytechnic Institute, 4105 Center for Biotechnology & Interdisciplinary StudiesTroyUSA
  4. 4.Department of Chemical and Biological Engineering, Department of BiologyRensselaer Polytechnic Institute, 2213 Center for Biotechnology & Interdisciplinary StudiesTroyUSA

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