Abstract
The sequential production of bioethanol and lactic acid from starch materials and lignocellulosic materials was investigated as ethanol fermentation broth (EFB) can provide nutrients for lactic acid bacteria. A complete process was developed, and all major operations are discussed, including ethanol fermentation, broth treatment, lactic acid fermentation, and product separation. The effect of process parameters, including ethanol fermentation conditions, treatment methods, and the amount of EFB used in simultaneous saccharification and fermentation (SSF), is investigated. Under the selected process conditions, the integrated process without additional chemical consumption provides a 1.08 acid/alcohol ratio (the broth containing 22.4 g/L ethanol and 47.6 g/L lactic acid), which corresponds to a polysaccharide utilization ratio of 86.9 %. Starch ethanol can thus promote cellulosic lactic acid by providing important nutrients for lactic acid bacteria, and in turn, cellulosic lactic acid could promote starch ethanol by improving the profit of the ethanol production process. Two process alternatives for the integration of starch ethanol and cellulosic lactic acid are compared, and some suggestions are given regarding the reuse of yeast following the cellulosic SSF step for lactic acid production.
Similar content being viewed by others
References
Altaf M, Venkateshwar M, Srijana M, Reddy G (2007) An economic approach for L-(+) lactic acid fermentation by Lactobacillus amylophilus GV6 using inexpensive carbon and nitrogen sources. J Appl Microbiol 103:372–380
Anders W, Mats G, Guido Z (2003) Techno-economic evaluation of producing ethanol from softwood: comparison of ssf and shf and identification of bottlenecks. Biotechnol Prog 19:1109–1117
Aparicio S, Alcalde R (2009) Insights into the ethyl lactate plus water mixed solvent. J Phys Chem B 113:14257–14269
Bustos G, Moldes AB, Cruz JM, Dominguez JM (2004) Evaluation of vinification lees as a general medium for Lactobacillus strains. J Agric Food Chem 52:5233–5239
Bustos G, Moldes AB, Cruz JM, Dominguez JM (2005) Production of lactic acid from vine-trimming wastes and viticulture lees using a simultaneous saccharification fermentation method. J Sci Food Agric 85:466–472
Gao MT, Hirata M, Toorisaka E, Hano T (2006) Study on acid-hydrolysis of spent cells for lactic acid fermentation. Biochem Eng J 28:87–91
Gao MT, Hirata ME, Toorisaka E, Hano T (2007) Lactic acid production with the supplementation of spent cells and fish wastes for the purpose of reducing impurities in fermentation broth. Biochem Eng J 36:276–280
Gao MT, Kaneko M, Hirata M, Toorisaka E, Hano T (2008) Utilization of rice bran as nutrient source for fermentative lactic acid production. Bioresour Technol 99:3659–3664
John RP, Nampoothiri KM, Pandey A (2007) Fermentative production of lactic acid from biomass: an overview on process developments and future perspectives. Appl Microbiol Biotechnol 74:524–534
Kim S, Dale BE (2004) Global potential bioethanol production from wasted crops and crop residues. Biomass Bioenergy 26:361–375
Linde M, Galbe M, Zacchi G (2008) Bioethanol production from non-starch carbohydrate residues in process streams from a dry-mill ethanol plant. Bioresour Technol 99:6505–6511
Liu CB, Hu B, Chen S, Glass RW (2007) Utilization of condensed distillers solubles as nutrient supplement for production of nisin and lactic acid from whey. Appl Biochem Biotechnol 137:875–884
Lu ZD, He F, Shi Y, Lu MB, Yu LJ (2010) Fermentative production of L(+)-lactic acid using hydrolyzed acorn starch persimmon juice and wheat bran hydrolysate as nutrients. Bioresour Technol 101:3642–3648
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Moore W, Johnson D (1967) Procedures for the chemical analysis of wood and wood products. U.S. Forest Products Laboratory, U.S. Department of Agriculture, Madison
Rivas B, Moldes AB, Dominguez JM, Parajo JC (2004) Lactic acid production from corn cobs by simultaneous saccharification and fermentation: a mathematical interpretation. Enzyme Microb Technol 34:627–634
Sun R, Song XL, Sun RC, Jiang JX (2011) Effect of lignin content on enzymatic hydrolysis of furfural residues. Bioresources 6:317–328
Suna XH, Wang QH, Zhao WC, Ma HZ, Sakata K (2006) Extraction and purification of lactic acid from fermentation broth by esterification and hydrolysis method. Sep Purif Technol 49:43–48
Tang Y, Zhao DQ, Zhu LW, Jiang JX (2011a) Simultaneous saccharification and fermentation of furfural residues by mixed cultures of lactic acid bacteria and yeast to produce lactic acid and ethanol. Eur Food Res Technol 233:489–495
Tang Y, Zhao DQ, Cristhian C, Jiang JX (2011b) Simultaneous saccharification and cofermentation of lignocellulosic residues from commercial furfural production and corn kernels using different nutrient media. Biotechnol Biofuels 4:22
TAPPI (2006) TAPPI Test Method T222 om-06, Acid-insoluble lignin in wood and pulp. In: TAPPI test methods. Technical Association of the Pulp and Paper Industry, Atlanta
Yang CZ, Li Y, Ruan N, Mou DH, Kang ML (2006) Study and applications of technology about breaking yeast cell wall. Food Sci Technol 7:138–142
Yao HW, Guo SG, Fan YM (2005) Research report on technology of yeast cell disruption. China Brew 4:32–34
Zhang SQ, Kao GL, Wei B, Zhang GH, Dong LB (2008) The study of the effect for the shiver of the yeast cellusing acid-heat shiver. J Inn Mong Agric Univ 3:15–18
Zhao WJ, Sun XH, Wang QH, Ma HZ, Teng Y (2009) Lactic acid recovery from fermentation broth of kitchen garbage by esterification and hydrolysis method. Biomass Bioenerg 33:21–25
Acknowledgments
The authors are grateful for the financial support of this research from the Fundamental Research Funds for the Central Universities (BLYJ201212), National Science Foundation of China (31070510), and Major State Basic Research Projects of China (973-2010CB732204).
Conflict of interest
The authors declare that they have no competing interests.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tang, Y., Zhu, L., Zhang, W. et al. Integrated process of starch ethanol and cellulosic lactic acid for ethanol and lactic acid production. Appl Microbiol Biotechnol 97, 1923–1932 (2013). https://doi.org/10.1007/s00253-012-4461-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00253-012-4461-x