Abstract
A dibutyl phthalate (DBP) transforming bacterium, strain M673, was isolated and identified as Acinetobacter sp. This strain could not grow on dialkyl phthalates, including dimethyl, diethyl, dipropyl, dibutyl, dipentyl, dihexyl, di(2-ethylhexyl), di-n-octyl, and dinonyl phthalate, but suspensions of cells could transform these compounds to phthalate via corresponding monoalkyl phthalates. During growth in Luria–Bertani medium, M673 produced the high amounts of non-DBP-induced intracellular hydrolase in the stationary phase. One DBP hydrolase gene containing an open reading frame of 1,095 bp was screened from a genomic library, and its expression product hydrolyzed various dialkyl phthalates to the corresponding monoalkyl phthalates.
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Acknowledgments
This work was supported by the National Natural Science Foundation (Project nos. 21107047 and 31100087), the Fundamental Research Funds for Central Universities of The People’s Republic of China (Project nos. 1107021154 and 1118021114), and two grants (Project nos. Y3100018 and 2011C23065) from Zhejiang provincial government.
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Wu, J., Liao, X., Yu, F. et al. Cloning of a dibutyl phthalate hydrolase gene from Acinetobacter sp. strain M673 and functional analysis of its expression product in Escherichia coli . Appl Microbiol Biotechnol 97, 2483–2491 (2013). https://doi.org/10.1007/s00253-012-4232-8
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DOI: https://doi.org/10.1007/s00253-012-4232-8