The introduction of various iron-chelating agents to the Magnetospirillum magneticum strain AMB-1 bacterial growth medium stimulated the growth of M. magneticum strain AMB-1 magnetotactic bacteria and enhanced the production of magnetosomes. After 7 days of growth, the number of bacteria and the production of magnetosomes were increased in the presence of iron-chelating agents by factors of up to ∼2 and ∼6, respectively. The presence of iron-chelating agents also produced an increase in magnetosome size and chain length and yielded improved magnetosome heating properties. The specific absorption rate of suspensions of magnetosome chains isolated from M. magneticum strain AMB-1 magnetotactic bacteria, measured under the application of an alternating magnetic field of average field strength ∼20 mT and frequency 198 kHz, increased from ∼222 W/gFe in the absence of iron-chelating agent up to ∼444 W/gFe in the presence of 4 μM rhodamine B and to ∼723 W/gFe in the presence of 4 μM EDTA. These observations were made at an iron concentration of 20 μM and iron-chelating agent concentrations below 40 μM.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Alphandéry E, Ngo AT, Lefèvre C, Lisiecki I, Wu LF, Pileni MP (2008) Difference between the magnetic properties of the magnetotactic bacteria and those of the extracted magnetosomes: influence of the distance between the chains of magnetosomes. J Phys Chem 112:12304–12309
Alphandéry E, Faure S, Seksek O, Guyot F, Chebbi I (2011) Chains of magnetosomes extracted from AMB-1 magnetotactic bacteria for application in alternative magnetic field cancer therapy. ACS Nano 5:6279–6296
Arakaki A, Nakazawa H, Nemoto M, Mori T, Matsunaga T (2008) Formation of magnetite by bacteria and its application. J R Soc Interface 5:977–999
Bazylinski DA, Frankel RB (2004) Magnetosome formation in prokaryotes. Nat Rev Microbiol 2:217–230
Bordelon DE, Cornejo C, Grüttner C, Westphal F, DeWeese TL, Ivkov R (2011) Magnetic nanoparticle heating efficiency reveals magneto-structural differences when characterized with wide ranging and high amplitude alternating magnetic fields. J Appl Phys 109:124904
Calugay RJ, Miyashita H, Okamura Y, Matsunaga T (2003) Siderophore production by the magnetic bacterium Magnetospirillum magneticum AMB-1 FEMS. Microbiol Lett 218:371–375
Guo F, Liu Y, Chen Y, Tang T, Jiang W, Li Y, Li J (2011) A novel rapid and continuous procedure for large-scale purification of magnetosomes from Magnetospirillum gryphiswaldense. Appl Microbiol Biotechnol 90:1277–1283
Heyen U, Schüler D (2003) Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor. Appl Microbiol Biotechnol 61:536–544
Komeili A (2006) Cell biology of magnetosome formation. Microbiol Monogr 3:163–174
Kundu S, Kulkarni GR (2010) Enhancement of magnetotactic bacterial yield in a modified MSGM medium without alteration of magnetosomes properties. Indian J Exp Bio 48:518–523
Lang C, Schüler D (2006) Biogenic nanoparticles: production, characterization, and application of bacterial magnetosomes. J Phys Condens Matter 18:S2815–S2828
Liu Y, Li GR, Guo F, Jiang W, Li Y, Li LJ (2010) Large-scale production of magnetosomes by chemostat culture of Magnetospirillum gryphiswaldense at high cell density. Microbial Cell Factories 9:99
Matsunaga T, Tadokoro F, Nakamura N (1990) Mass culture of magnetic bacteria and their application to flow type immunoassays. IEE Trans Mag 26:1557–1559
Matsunaga T, Kawasaki M, Yu X, Tsujimura N, Nakamura N (1996a) Chemiluminescence enzyme immunoassay using bacterial magnetic particles. Anal Chem 68:3551–3554
Matsunaga T, Tsujimura N, Kamiya S (1996b) Enhancement of magnetic particle production by nitrate and succinate fed-batch culture of Magnetospirillum sp. AMB-1. Biotechnol Tech 10:495–500
Matsunaga T, Togo H, Tanaka T (2000) Production of luciferase-magnetic particle complex by recombinant Magnetospirillum sp. AMB-1. Biotechnol Bioeng 70:704–709
Reichard PU, Kretzschmar R, Kraemer SM (2007) Dissolution mechanisms of goethite in the presence of siderophores and organic acids. Geochim Cosmochim Acta 71:5635–5650
Schüler D, Bauerlein E (1996) Iron-limited growth and kinetics of iron uptake in Magnetospirillum gryphiswaldense. Arch Microbiol 166:301–307
Sun JB, Duan JH, Dai SL, Ren J, Zhang YD, Tim JS, Li Y (2007) In vitro and in vivo antitumor effects of doxorubicin loaded with bacterial magnetosomes (DBMS) on H22 cells: the magnetic bio-nanoparticles as drug carriers. Cancer Lett 258:109–117
Sun JB, Zhao F, Tang T, Jiang W, Tian JS, Li Y, Li JL (2008) High-yield growth and magnetosome formation by Magnetospirillum gryphiswaldense MSR-1 in an oxygen-controlled fermentor supplied solely with air. Appl Microbiol Biotechnol 79:389–397
Yang CD, Takeyama H, Tanaka T, Matsunaga T (2001a) Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1. Enz Microb Tech 29:13–19
Yang C, Takeyama H, Matsunaga T (2001b) Iron feeding optimization and plasmid stability in production of recombinant bacterial magnetic particles by Magnetospirillum magneticum AMB-1 in fed-batch culture. J Biosci Bioeng 2:213–216
Yang CD, Takeyama H, Tanaka T, Hasegawa A, Matsunaga T (2001c) Synthesis of bacterial magnetic particles during cell cycle of Magnetospirillum magneticum AMB-1. Appl Biochem Biotech 91:155–160
F. Guyot and M. Amor claim having no inventive contribution to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
(DOCX 1603 kb)
About this article
Cite this article
Alphandéry, E., Amor, M., Guyot, F. et al. The effect of iron-chelating agents on Magnetospirillum magneticum strain AMB-1: stimulated growth and magnetosome production and improved magnetosome heating properties. Appl Microbiol Biotechnol 96, 663–670 (2012). https://doi.org/10.1007/s00253-012-4199-5
- Magnetotactic bacteria
- Iron-chelating agents
- Magnetic hyperthermia
- Alternating magnetic field