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Iodine from bacterial iodide oxidization by Roseovarius spp. inhibits the growth of other bacteria

Applied Microbiology and Biotechnology volume 97, pages 2173–2182 (2013)Cite this article

Abstract

Microbial activities in brine, seawater, or estuarine mud are involved in iodine cycle. To investigate the effects of the microbiologically induced iodine on other bacteria in the environment, a total of 13 bacteria that potentially participated in the iodide-oxidizing process were isolated from water or biofilm at a location containing 131 μg ml−1 iodide. Three distinct strains were further identified as Roseovarius spp. based on 16 S rRNA gene sequences after being distinguished by restriction fragment length polymorphism analysis. Morphological characteristics of these three Roseovarius spp. varied considerably across and within strains. Iodine production increased with Roseovarius spp. growth when cultured in Marine Broth with 200 μg ml−1 iodide (I−). When 106 CFU/ml Escherichia coli, Pseudomonas aeruginosa, and Bacillus pumilus were exposed to various concentrations of molecular iodine (I2), the minimum inhibitory concentrations (MICs) were 0.5, 1.0, and 1.0 μg ml−1, respectively. However, fivefold increases in the MICs for Roseovarius spp. were obtained. In co-cultured Roseovarius sp. IOB-7 and E. coli in Marine Broth containing iodide (I−), the molecular iodine concentration was estimated to be 0.76 μg ml−1 after 24 h and less than 50 % of E. coli was viable compared to that co-cultured without iodide. The growth inhibition of E. coli was also observed in co-cultures with the two other Roseovarius spp. strains when the molecular iodine concentration was assumed to be 0.52 μg ml−1.

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Acknowledgments

The authors are grateful to Japan Oil, Gas and Metals National Corporation for financial support for this research and to Mr. Jun Koki from the Centre for Advanced Materials, Tokyo Institute of Technology for technical support with the SEM imaging.

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Authors and Affiliations

  1. Department of Bioengineering, Tokyo Institute of Technology, 4259 J2-15 Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan

    Dan Zhao, Choon-Ping Lim, Kazuhiko Miyanaga & Yasunori Tanji

  2. College of Materials Science and Chemical Engineering, Harbin Engineering University, No. 145 Nantong Street, Harbin, Heilongjiang, 150001, People’s Republic of China

    Dan Zhao

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  1. Dan Zhao
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  2. Choon-Ping Lim
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  3. Kazuhiko Miyanaga
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  4. Yasunori Tanji
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Correspondence to Yasunori Tanji.

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Zhao, D., Lim, CP., Miyanaga, K. et al. Iodine from bacterial iodide oxidization by Roseovarius spp. inhibits the growth of other bacteria. Appl Microbiol Biotechnol 97, 2173–2182 (2013). https://doi.org/10.1007/s00253-012-4043-y

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  • DOI: https://doi.org/10.1007/s00253-012-4043-y

Keywords

  • Iodide-oxidizing process
  • Molecular iodine
  • Roseovarius spp.
  • Minimum inhibitory concentration
  • Growth inhibition