Applied Microbiology and Biotechnology

, Volume 97, Issue 2, pp 881–889 | Cite as

Effective bioremoval and detoxification of textile dye mixture by Alishewanella sp. KMK6

  • Yogesh M. Kolekar
  • Pallavi D. Konde
  • Vijay L. Markad
  • Sharwari V. Kulkarni
  • Ashvini U. Chaudhari
  • Kisan M. KodamEmail author
Environmental biotechnology


Alishewanella sp. strain KMK6 was able to degrade mixture of textile dyes (0.5–2.0 g l−1) within 8 h. An initial 28 % reduction in COD was observed immediately after decolorization at static anoxic conditions which on further incubation at shaking conditions reduced by 90 %. Partially purified azoreductase was able to utilize different azo dyes as substrates. The HPLC profile of dye degradation showed formation of metabolic products. Further FTIR analysis showed significant changes in the peaks corresponding to functional groups present in dye mixture and its degradation products. The genotoxicity assessment showed that the dye degradation products were non-toxic compared to dye mixture.


Alishewanella sp. KMK6 Azoreductase Biodegradation Azo dyes COD 



The authors, YMK and VLM, thank the University Grants Commission (UGC), New Delhi, India for research fellowship.


  1. Anastasi A, Spina F, Prigione V, Tigini V, Giansanti P, Varese GC (2010) Scale-up of a bioprocess for textile wastewater treatment using Bjerkandera adusta. Bioresour Technol 101:3067–3075CrossRefGoogle Scholar
  2. APHA (1998) Standard method for the examination of water and wastewater, 20th edn. American public health association, 2120 E, Washington, DC, USAGoogle Scholar
  3. Bechtold T, Turcanu A (2006) Iron-complexes of bis(2-hydroxyethyl)-amino-compounds as mediators for the indirect reduction of dispersed vat dyes-cyclic voltammetry and spectroelectrochemical experiments. J Electroanal Chem 591:118–126CrossRefGoogle Scholar
  4. Bozic M, Pricelius S, Guebitz GM, Kokol V (2010) Enzymatic reduction of complex redox dyes using NADH-dependent reductase from Bacillus subtilis coupled with cofactor regeneration. Appl Microbiol Biotechnol 85:563–571CrossRefGoogle Scholar
  5. Champagne PP, Nesheim ME, Ramsay JA (2010) Effect of a non-ionic surfactant, Merpol, on dye decolorization of reactive blue 19 by laccase. Enzyme Microb Technol 46:147–152CrossRefGoogle Scholar
  6. Chan GF, Abdul Rashid NA, Chua LS, Ab.llah N, Nasiri R, Mohamad Ikubar MR (2012) Communal microaerophilic–aerobic biodegradation of Amaranth by novel NAR-2 bacterial consortium. Bioresour Technol 105:48–59CrossRefGoogle Scholar
  7. Chen H (2006) Recent advances in azo dye degrading enzyme research. Curr Protein Pept Sci 7:101–111CrossRefGoogle Scholar
  8. Chen H, Hopper S, Cerniglia C (2005) Biochemical and molecular characterization of an azoreductase from Staphylococcus aureus, a tetrameric NADPH-dependent flavoprotein. Microbiology 151:1433–1441CrossRefGoogle Scholar
  9. Dong X, Zhou J, Liu Y (2003) Peptone-induced biodecolorization of reactive brilliant blue (KN-R) by Rhodocyclus gelatinosus XL-1. Process Biochem 39:89–94CrossRefGoogle Scholar
  10. Elisangela F, Andrea Z, Fabio DG, Cristiano RM, Regina DL, Artur CP (2009) Biodegradation of textile azo dyes by a facultative Staphylococcus arlettae strain VN-11 using a sequential microaerophilic/aerobic process. Int Biodeterior Biodegrad 63:280–288CrossRefGoogle Scholar
  11. Galai S, Limam F, Marzouki MN (2010) Decolorization of an industrial effluent by free and immobilized cells of Stenotrophomonas maltophilia AAP56. Implementation of efficient down flow column reactor. World J Microbiol Biotechnol 26:1341–1347CrossRefGoogle Scholar
  12. Gomare SS, Tamboli DP, Kagalkar AN, Govindwar SP (2009) Eco-friendly biodegradation of a reactive textile dye golden yellow HER by Brevibacillus laterosporus MTCC 2298. Int Biodeterior Biodegrad 63:582–586CrossRefGoogle Scholar
  13. Gopinath KP, Asan Meera Sahib H, Muthukumar K, Velan M (2009) Improved biodegradation of congo red by using Bacillus sp. Bioresour Technol 100:670–675CrossRefGoogle Scholar
  14. Hai FI, Yamamoto K, Fukushi K (2007) Hybrid treatment system for dye wastewater. Crit Rev Environ Sci Technol 37:315–377CrossRefGoogle Scholar
  15. Jang MS, Jung BG, Sung NC, Lee YC (2007) Decolorization of textile plant effluent by Citrobacter sp. strain KCTC 18061P. J Gen Appl Microbiol 53:339–343CrossRefGoogle Scholar
  16. Kalme SD, Jadhav SU, Parshetti GK, Govindwar SP (2010) Biodegradation of green HE4B: co-substrate effect, biotransformation enzymes and metabolite toxicity analysis. Indian J Microbiol 50:156–164CrossRefGoogle Scholar
  17. Kalyani DC, Patil PS, Jadhav JP, Govindwar SP (2008) Biodegradation of reactive textile dye red BLI by an isolated bacterium Pseudomonas sp. SUK1. Bioresour Technol 99:4635–4641CrossRefGoogle Scholar
  18. Klepacz-Smolka A, Sojka-Ledakowicz J, Pazdzior K, Ledakowicz S (2010) Application of anoxic fixed film and aerobic CSTR bioreactor in treatment of nanofiltration concentrate of real textile wastewater. Chem Pap 64:230–236CrossRefGoogle Scholar
  19. Kolekar YM, Kodam KM (2011) Decolorization of textile dyes by Alishewanella sp. KMK6. Appl Microbiol Biotechnol. doi: 10.1007/s00253-011-3698-0
  20. Kolekar YM, Nemade HN, Markad VL, Adav SS, Patole MS, Kodam KM (2012) Decolorization and biodegradation of azo dye, reactive blue 59 by aerobic granules. Bioresour Technol 104:818–822CrossRefGoogle Scholar
  21. Lin J, Zhang X, Li Z, Lei L (2010) Biodegradation of reactive blue 13 in a two-stage anaerobic/aerobic fluidized beds system with a Pseudomonas sp. isolate. Bioresour Technol 101:34–40CrossRefGoogle Scholar
  22. Lodato A, Alfierib F, Olivieri G, Di Donato A, Marzocchella A, Salatino P (2007) Azo-dye conversion by means of Pseudomonas sp. OX1. Enzyme Microb Technol 41:646–652CrossRefGoogle Scholar
  23. Machado K, Compart L, Morais R, Ros L, Santos M (2006) Biodegradation of reactive textile dyes by basidiomycetous fungi from Brazilian ecosystems. Braz J Microbiol 37:481–487CrossRefGoogle Scholar
  24. Maier J, Kandelbaner A, Eracher A, Cavaco-Paulo A, Gubitz GM (2004) A new alkali-thermostable azoreductase from Bacillus sp. strain SF. Appl Environ Microbiol 70:837–844CrossRefGoogle Scholar
  25. Moawad H, El-Rahim WM, Khalafallah M (2003) Evaluation of biotoxicity of textile dyes using two bioassays. J Basic Microbiol 43:218–229CrossRefGoogle Scholar
  26. Pathak H, Patel S, Rathod M, Chauhan K (2011) In vitro studies on degradation of synthetic dye mixture by Comamonas sp. VS-MH2 and evaluation of its efficacy using simulated microcosm. Bioresour Technol 102:10391–10400CrossRefGoogle Scholar
  27. Phugare SS, Kalyani DC, Patil AV, Jadhav JP (2011) Textile dye degradation by bacterial consortium and subsequent toxicological analysis of dye and dye metabolites using cytotoxicity, genotoxicity and oxidative stress studies. J Hazard Mater 186:713–723CrossRefGoogle Scholar
  28. Robinson T, McMullan G, Marchant R, Nigam P (2001) Remediation of dyes in textile effluent—a critical review on current treatment technologies with a proposed alternative. Bioresour Technol 77:247–255CrossRefGoogle Scholar
  29. Senan RC, Abraham TE (2004) Bioremediation of textile azo dyes by aerobic bacterial consortium. Biodegradation 15:275–280CrossRefGoogle Scholar
  30. Sharma P, Singh L, Dilbaghi N (2009) Response surface methodological approach for the decolorization of simulated dye effluent using Aspergillus fumigatus fresenius. J Hazard Mater 161:1081–1086CrossRefGoogle Scholar
  31. Tamboli DP, Gomare SS, Kalme SS, Jadhav UU, Govindwar SP (2010) Degradation of orange 3R, mixture of dyes and textile effluent and production of polyhydroxy alkanoates from biomass obtained after degradation. Int Biodeterior Biodegrad 64:755–763CrossRefGoogle Scholar
  32. Telke AA, Joshi SM, Jadhav SU, Tamboli DP, Govindwar SP (2010) Decolorization and detoxification of congo red and textile industry effluent by an isolated bacterium Pseudomonas sp. SU-EBT. Biodegradation 21:283–296CrossRefGoogle Scholar
  33. Wakeyama H, Takeshige K, Minakami S (1983) NADPH-dependent reduction of 2,6-dichlorophenol-indophenol by the phagocytic vesicles of pig polymorphonuclear leucocytes. Biochem J 210:577–581Google Scholar
  34. Zhang MM, Chen WM, Chen BY, Chang CT, Hsueh CC, Ding Y, Lin KL, Xu H (2010) Comparative study on characteristics of azo dye decolorization by indigenous decolorizers. Bioresour Technol 101:2651–2656CrossRefGoogle Scholar
  35. Zimmerman T, Kulla HG, Leisinger T (1982) Properties of purified orange II azo reductase, the enzyme initiating azo dye degradation by Pseudomonas KF46. Eur J Biochem 129:197–203CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Yogesh M. Kolekar
    • 1
  • Pallavi D. Konde
    • 1
  • Vijay L. Markad
    • 1
  • Sharwari V. Kulkarni
    • 1
  • Ashvini U. Chaudhari
    • 1
  • Kisan M. Kodam
    • 1
    Email author
  1. 1.Biochemistry Division, Department of ChemistryUniversity of PunePuneIndia

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