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Production of 10-hydroxystearic acid from oleic acid and olive oil hydrolyzate by an oleate hydratase from Lysinibacillus fusiformis

An Erratum to this article was published on 02 June 2012

Abstract

A recombinant enzyme from Lysinibacillus fusiformis was expressed, purified, and identified as an oleate hydratase because the hydration activity of the enzyme was the highest for oleic acid (with a k cat of 850 min−1 and a K m of 540 μM), followed by palmitoleic acid, γ-linolenic acid, linoleic acid, myristoleic acid, and α-linolenic acid. The optimal reaction conditions for the enzymatic production of 10-hydroxystearic acid were pH 6.5, 35 °C, 4% (v/v) ethanol, 2,500 U ml−1 (8.3 mg ml−1) of enzyme, and 40 g l−1 oleic acid. Under these conditions, 40 g l−1 (142 mM) oleic acid was converted into 40 g l−1 (133 mM) 10-hydroxystearic acid for 150 min, with a molar yield of 94% and a productivity of 16 g l−1 h−1, and olive oil hydrolyzate containing 40 g l−1 oleic acid was converted into 40 g l−1 10-hydroxystearic acid for 300 min, with a productivity of 8 g l−1 h−1.

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Acknowledgment

This study was supported by a grant (Code#000407980110) from Small and Medium Business Administration, Republic of Korea.

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Correspondence to Deok-Kun Oh.

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Kim, BN., Joo, YC., Kim, YS. et al. Production of 10-hydroxystearic acid from oleic acid and olive oil hydrolyzate by an oleate hydratase from Lysinibacillus fusiformis . Appl Microbiol Biotechnol 95, 929–937 (2012). https://doi.org/10.1007/s00253-011-3805-2

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  • DOI: https://doi.org/10.1007/s00253-011-3805-2

Keywords

  • Lysinibacillus fusiformis
  • Oleic acid
  • 10-Hydroxystearic acid production
  • Oleate hydratase
  • Olive oil hydrolyzate