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Applied Microbiology and Biotechnology

, Volume 93, Issue 3, pp 975–982 | Cite as

Best practices for fungal germplasm repositories and perspectives on their implementation

  • Aric Wiest
  • Robert Schnittker
  • Mike Plamann
  • Kevin McCluskey
Mini-Review

Abstract

In over 50 years, the Fungal Genetics Stock Center has grown to become a world-recognized biological resource center. Along with this growth comes the development and implementation of myriad practices for the management and curation of a diverse collection of filamentous fungi, yeast, and molecular genetic tools for working with the fungi. These practices include techniques for the testing, manipulation, and preservation of individual fungal isolates as well as for processing of thousands of isolates in parallel. In addition to providing accurate record keeping, an electronic managements system allows the observation of trends in strain distribution and in sample characteristics. Because many ex situ fungal germplasm repositories around the world share similar objectives, best-practice guidelines have been developed by a number of organizations such as the Organization for Economic Cooperation and Development or the International Society for Biological and Environmental Repositories. These best-practice guidelines provide a framework for the successful operation of collections and promote the development and interactions of biological resource centers around the world.

Keywords

Biological resource center Neurospora Cryopreservation Lyophilization L-drying High-throughput screening 

Notes

Acknowledgments

The Fungal Genetics Stock Center is supported by Grant #742713 from the US National Science Foundation.

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Aric Wiest
    • 1
  • Robert Schnittker
    • 1
  • Mike Plamann
    • 1
  • Kevin McCluskey
    • 1
  1. 1.Fungal Genetics Stock Center, School of Biological SciencesUniversity of Missouri-Kansas CityKansas CityUSA

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