Applied Microbiology and Biotechnology

, Volume 93, Issue 6, pp 2309–2316 | Cite as

Continuous bio-catalytic conversion of sugar mixture to acetone–butanol–ethanol by immobilized Clostridium acetobutylicum DSM 792

  • Shrikant A. Survase
  • Adriaan van Heiningen
  • Tom GranströmEmail author
Biotechnological products and process engineering


Continuous production of acetone, n-butanol, and ethanol (ABE) was carried out using immobilized cells of Clostridium acetobutylicum DSM 792 using glucose and sugar mixture as a substrate. Among various lignocellulosic materials screened as a support matrix, coconut fibers and wood pulp fibers were found to be promising in batch experiments. With a motive of promoting wood-based bio-refinery concept, wood pulp was used as a cell holding material. Glucose and sugar mixture (glucose, mannose, galactose, arabinose, and xylose) comparable to lignocellulose hydrolysate was used as a substrate for continuous production of ABE. We report the best solvent productivity among wild-type strains using column reactor. The maximum total solvent concentration of 14.32 g L−1 was obtained at a dilution rate of 0.22 h−1 with glucose as a substrate compared to 12.64 g L−1 at 0.5 h−1 dilution rate with sugar mixture. The maximum solvent productivity (13.66 g L−1 h−1) was obtained at a dilution rate of 1.9 h−1 with glucose as a substrate whereas solvent productivity (12.14 g L−1 h−1) was obtained at a dilution rate of 1.5 h−1 with sugar mixture. The immobilized column reactor with wood pulp can become an efficient technology to be integrated with existing pulp mills to convert them into wood-based bio-refineries.


n-Butanol Wood pulp Immobilization Clostridium acetobutylicum 



This study belongs to the project of Commodity Chemicals from Forest Biomass—Bioforest, which is part of the Biorefine technology program financed by Tekes (The Finnish Funding Agency for Technology and Innovation, Finland). In addition, the financial support from industrial collaborators is greatly acknowledged.


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Shrikant A. Survase
    • 1
  • Adriaan van Heiningen
    • 2
  • Tom Granström
    • 1
    Email author
  1. 1.Department of Biotechnology and Chemical TechnologyAalto University School of Chemical EngineeringAaltoFinland
  2. 2.Department of Chemical and Biological EngineeringUniversity of MaineOronoUSA

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