Applied Microbiology and Biotechnology

, Volume 93, Issue 3, pp 917–930

CHO cells in biotechnology for production of recombinant proteins: current state and further potential


DOI: 10.1007/s00253-011-3758-5

Cite this article as:
Kim, J.Y., Kim, YG. & Lee, G.M. Appl Microbiol Biotechnol (2012) 93: 917. doi:10.1007/s00253-011-3758-5


Recombinant Chinese hamster ovary cells (rCHO) cells have been the most commonly used mammalian host for large-scale commercial production of therapeutic proteins. Recent advances in cell culture technology for rCHO cells have achieved significant improvement in protein production leading to titer of more than 10 g/L to meet the huge demand from market needs. This achievement is associated with progression in the establishment of high and stable producer and the optimization of culture process including media development. In this review article, we focus on current strategies and achievements in cell line development, mainly in vector engineering and cell engineering, for high and stable protein production in rCHO cells. The approaches that manipulate various DNA elements for gene targeting by site-specific integration and cis-acting elements to augment and stabilize gene expression are reviewed here. The genetic modulation strategy by “direct” cell engineering with growth-promoting and/or productivity-enhancing factors and omics-based approaches involved in transcriptomics, proteomics, and metabolomics to pursue cell engineering are also presented.


CHO cells Cell line development Vector engineering Cell engineering Omics-based approaches 

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Biological SciencesGraduate School of Nanoscience & Technology (WCU), KAISTDaejeonKorea
  2. 2.Biotechnology Process Engineering Center, KRIBBDaejeonKorea

Personalised recommendations