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Mannitol production by heterofermentative Lactobacillus reuteri CRL 1101 and Lactobacillus fermentum CRL 573 in free and controlled pH batch fermentations

  • Applied microbial and cell physiology
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Abstract

Certain lactic acid bacteria, especially heterofermentative strains, are capable to produce mannitol under adequate culture conditions. In this study, mannitol production by Lactobacillus reuteri CRL 1101 and Lactobacillus fermentum CRL 573 in modified MRS medium containing a mixture of fructose and glucose in a 6.5:1.0 ratio was investigated during batch fermentations with free pH and constant pH 6.0 and 5.0. Mannitol production and yields were higher under constant pH conditions compared with fermentations with free pH, the increase being more pronounced in the case of the L. fermentum strain. Maximum mannitol production and yields from fructose for L. reuteri CRL 1101 (122 mM and 75.7 mol%, respectively) and L. fermentum CRL 573 (312 mM and 93.5 mol%, respectively) were found at pH 5.0. Interestingly, depending on the pH conditions, fructose was used only as an alternative external electron acceptor or as both electron acceptor and energy source in the case of the L. reuteri strain. In contrast, L. fermentum CRL 573 used fructose both as electron acceptor and carbon source simultaneously, independently of the pH value, which strongly affected mannitol production by this strain. Studies on the metabolism of these relevant mannitol-producing lactobacilli provide important knowledge to either produce mannitol to be used as food additive or to produce it in situ during fermented food production.

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Acknowledgments

We acknowledge the financial support of CONICET, FONCyT, and MINCyT from Argentina and FWO-Flanders from Belgium (bilateral scientific cooperation project FWO/06/01), the cultural agreement between CONICET-Argentina and IMDO-Vrije Universiteit Brussel, and the Research Council of the Vrije Universiteit Brussel.

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Authors and Affiliations

  1. Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, San Miguel de Tucumán, 4000, Argentina

    Cecilia Rodríguez, María José Fornaguera, Graciela Font de Valdez & Fernanda Mozzi

  2. Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Faculty of Sciences and Bio-engineering Sciences, Vrije Universiteit Brussel (VUB), Pleinlaan 2, 1050, Brussels, Belgium

    Cecilia Rodríguez, Tom Rimaux, Gino Vrancken & Luc De Vuyst

Authors
  1. Cecilia Rodríguez
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  2. Tom Rimaux
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  3. María José Fornaguera
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  4. Gino Vrancken
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  5. Graciela Font de Valdez
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  6. Luc De Vuyst
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  7. Fernanda Mozzi
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Correspondence to Fernanda Mozzi.

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Rodríguez, C., Rimaux, T., Fornaguera, M.J. et al. Mannitol production by heterofermentative Lactobacillus reuteri CRL 1101 and Lactobacillus fermentum CRL 573 in free and controlled pH batch fermentations. Appl Microbiol Biotechnol 93, 2519–2527 (2012). https://doi.org/10.1007/s00253-011-3617-4

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  • DOI: https://doi.org/10.1007/s00253-011-3617-4

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