Applied Microbiology and Biotechnology

, Volume 93, Issue 2, pp 787–796 | Cite as

Distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria Ochrobactrum anthropi and Achromobacter sp

  • Alexey V. Sviridov
  • Tatyana V. Shushkova
  • Nina F. Zelenkova
  • Natalya G. Vinokurova
  • Igor G. Morgunov
  • Inna T. Ermakova
  • Alexey A. Leontievsky
Applied microbial and cell physiology


Bacterial strains capable of utilizing methylphosphonic acid (MP) or glyphosate (GP) as the sole sources of phosphorus were isolated from soils contaminated with these organophosphonates. The strains isolated from MP-contaminated soils grew on MP and failed to grow on GP. One group of the isolates from GP-contaminated soils grew only on MP, while the other one grew on MP and GP. Strains Achromobacter sp. MPS 12 (VKM B-2694), MP degraders group, and Ochrobactrum anthropi GPK 3 (VKM B-2554D), GP degraders group, demonstrated the best degradative capabilities towards MP and GP, respectively, and were studied for the distribution of their organophosphonate catabolism systems. In Achromobacter sp. MPS 12, degradation of MP was catalyzed by C–P lyase incapable of degrading GP (C–P lyase I). Adaptation to growth on GP yielded the strain Achromobacter sp. MPS 12A, which retained its ability to degrade MP via C–P lyase I and was capable of degrading GP with formation of sarcosine, thus suggesting the involvement of a GP-specific C–P lyase II. O. anthropi GPK 3 also degraded MP via C–P lyase I, but degradation of GP in it was initiated by glyphosate oxidoreductase, which was followed by product transformation via the phosphonatase pathway.


Phosphonates Glyphosate Methylphosphonic acid C–P lyase Glyphosate Oxidoreductase Phosphonatase 



This work was supported by the International Science and Technology Center (Grant No. 1892.2), the Russian Federal Agency for Education (Grant No., and the Russian Foundation for Basic Research (Project No. 09-04-00320).


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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Alexey V. Sviridov
    • 1
  • Tatyana V. Shushkova
    • 1
  • Nina F. Zelenkova
    • 1
    • 2
  • Natalya G. Vinokurova
    • 1
  • Igor G. Morgunov
    • 1
  • Inna T. Ermakova
    • 1
    • 2
  • Alexey A. Leontievsky
    • 1
    • 2
    • 3
  1. 1.G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of SciencesPushchinoRussia
  2. 2.Pushchino State UniversityPushchinoRussia
  3. 3.IBPM RASPushchinoRussia

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