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Use of soybean vinasses as a germinant medium for a Geobacillus stearothermophilus ATCC 7953 sterilization biological indicator

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Abstract

A novel low-cost medium was developed from by-products and wastes from the ethanol agro-industry to replace commercial media in the production of a steam sterilization biological indicator (BI). Various recovery media were developed using soybean or sugarcane molasses and vinasse to prepare a self-contained BI. Media performance was evaluated by viability and heat resistance (D 121 °C value) according to regulatory standards. A medium produced with a soybean vinasse ratio of 1:70 (1.4%) (w/v) produced the results, with D 121 °C = 2.9 ± 0.5 min and Usk = 12.7 ± 2.1 min. The addition of 0.8% (w/v) yeast extract improved the germination of heat-damaged spores. The pH variation from 6.0 to 7.3 resulted in a gradual increase in the D 121 °C value. The absence of calcium chloride resulted in a decrease in germination, while no significant differences were observed with starch addition. Soybean vinasses may thus be used as the main component of a culture medium to substitute for commercial media in the production of self-contained biological indicators. The use of ethanol production waste in this biotechnological process realized a reliable performance, minimized the environmental impact, and decreased BI production costs while producing a high quality product.

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Acknowledgments

The present research was financially supported by the Secretaria de Estado da Ciência, Tecnologia e Ensino Superior-Fundo Paraná. The authors thank Neuza Araújo and Elza Retka for laboratory support.

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Authors and Affiliations

  1. Centro de Produção e Pesquisa de Imunobiológicos, Secretaria de Estado da Saúde do Paraná, Av. São Roque, 716, CEP 83.302-200, Piraquara, Paraná, Brazil

    Regina E. F. Dlugokenski, Sandra R. B. R. Sella & João C. Minozzo

  2. Divisão de Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná, Rua Cel. Francisco H. dos Santos, 100, CEP 81.531-990, Curitiba, Paraná, Brazil

    Regina E. F. Dlugokenski, Sandra R. B. R. Sella, Belquis P. Guizelini, Luciana P. S. Vandenberghe, Adenise L. Woiciechowski, Carlos R. Soccol & João C. Minozzo

  3. Rua Antonio Rebelatto, 122, CEP 81.710-120, Curitiba, Paraná, Brazil

    Regina E. F. Dlugokenski

Authors
  1. Regina E. F. Dlugokenski
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  2. Sandra R. B. R. Sella
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  3. Belquis P. Guizelini
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  4. Luciana P. S. Vandenberghe
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  5. Adenise L. Woiciechowski
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  6. Carlos R. Soccol
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  7. João C. Minozzo
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Correspondence to Regina E. F. Dlugokenski.

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Dlugokenski, R.E.F., Sella, S.R.B.R., Guizelini, B.P. et al. Use of soybean vinasses as a germinant medium for a Geobacillus stearothermophilus ATCC 7953 sterilization biological indicator. Appl Microbiol Biotechnol 90, 713–719 (2011). https://doi.org/10.1007/s00253-011-3160-3

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  • DOI: https://doi.org/10.1007/s00253-011-3160-3

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