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Construction of stress-induced metabolic pathway from glucose to 1,3-propanediol in Escherichia coli

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Abstract

1,3-Propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive synthesis. The aim of this study was to engineer an Escherichia coli strain that can produce 1,3-propanediol directly from glucose. We successfully constructed a stress-induced metabolic pathway from glucose to 1,3-propanediol in recombinant E. coli by the expression of gpd1 and gpp2 genes from Saccharomyces cerevisiae and dha operon from Klebsiella pneumoniae, respectively. Batch cultivation of the recombinant E. coli showed that 12.1 g/L 1,3-propanediol was accumulated in the culture without using any inducer.

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Acknowledgments

This work was financially supported by a grant from the National Natural Science Foundation of China (30870022) and a grant of the National Basic Research Program of China (2011CB707405).

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Correspondence to Qingsheng Qi.

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S. Figure 1

SDS-PAGE analysis of GPD1, GPP2, and fusion protein. Electrophoresis in 12% polyacrylamide gel. 1 E. coli DH5α/pSKGF which contains the fusion protein. 2 E. coli DH5α/pSKG which contains the respective GPD1 and GPP2. 3 E. coli DH5α. M protein marker (DOC 143 kb)

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Liang, Q., Zhang, H., Li, S. et al. Construction of stress-induced metabolic pathway from glucose to 1,3-propanediol in Escherichia coli . Appl Microbiol Biotechnol 89, 57–62 (2011). https://doi.org/10.1007/s00253-010-2853-3

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  • DOI: https://doi.org/10.1007/s00253-010-2853-3

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