Applied Microbiology and Biotechnology

, Volume 88, Issue 6, pp 1413–1422 | Cite as

Response of microbial community structure to microbial plugging in a mesothermic petroleum reservoir in China

Environmental Biotechnology

Abstract

Microbial plugging, a microbial enhancement of oil recovery (MEOR) technique, has been applied in a candidate oil reservoir of Daqing Oil Field (China). The goal of this study is to monitor the survival of injected bacteria and reveal the response of microbial communities in field trial of microbial plugging through injection of selected microbial culture broth and nutrients. Culture-dependent enrichment and culture-independent 16S rDNA clone library methods were used. The results show that it was easy to activate targeted biopolymer-producing bacteria in a laboratory environment, and it was difficult for injected exogenous bacteria to survive. In addition, microbial communities in the oil reservoir also changed before and after the field trial. However, microbial communities, activated by fermentative medium for biopolymer-producing bacteria, appeared to show greater differences in the laboratory than in the natural reservoir. It was concluded that microbial populations monitoring was important to MEOR; results of response of microbial communities could provide a guide for the future field trials.

Keywords

Microbial plugging Microbial enhancement of oil recovery (MEOR) Microbial communities Biopolymer-producing bacteria 16S rDNA clone library 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.The Key Laboratory of Marine Reservoir Evolution and Hydrocarbon Accumulation Mechanism, Ministry of Education, School of Energy Resources, China University of Geosciences (Beijing)BeijingChina
  2. 2.College of Chemistry and Molecular SciencesWuhan UniversityWuhanChina
  3. 3.College of Chemistry and Environmental EngineeringYangtze UniversityJingzhouChina
  4. 4.School of Biomedical SciencesUniversity of UlsterColeraineUK

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