Applied Microbiology and Biotechnology

, Volume 88, Issue 1, pp 341–358 | Cite as

Marine bacterial isolates inhibit biofilm formation and disrupt mature biofilms of Pseudomonas aeruginosa PAO1

  • Chari Nithya
  • Mansur Farzana Begum
  • Shunmugiah Karutha PandianEmail author
Environmental Biotechnology


According to the Centers for Disease Control and Prevention, biofilms cause 65% of infections in developed countries. Pseudomonas aeruginosa biofilm cause life threatening infections in cystic fibrosis infection and they are 1,000 times more tolerant to antibiotic than the planktonic cells. As quorum sensing, hydrophobicity index and extracellular polysaccharide play a crucial role in biofilm formation, extracts from 46 marine bacterial isolates were screened against these factors in P. aeruginosa. Eleven extracts showed antibiofilm activity. Extracts of S6-01 (Bacillus indicus = MTCC 5559) and S6-15 (Bacillus pumilus = MTCC 5560) inhibited the formation of PAO1 biofilm up to 95% in their Biofilm Inhibitory Concentration(BIC) of 50 and 60 μg/ml and 85% and 64% in the subinhibitory concentrations (1/4 and 1/8 of the BIC, respectively). Furthermore, the mature biofilm was disrupted to 70–74% in their BIC. The antibiofilm compound from S6-15 was partially purified using solvent extraction followed by TLC and silica column and further characterized by IR analysis. Current study for the first time reveals the antibiofilm and antiquorum-sensing activity of B. pumilus, B. indicus, Bacillus arsenicus, Halobacillus trueperi, Ferrimonas balearica, and Marinobacter hydrocarbonoclasticus from marine habitat.


PAO1 biofilm Antibiofilm Marine bacteria B. pumilus Hydrophobicity index Quorum sensing 



The authors gratefully acknowledge the computational and bioinformatics facility provided by the Alagappa University Bioinformatics Infrastructure Facility (funded by DBT, GOI; Grant No. BT/BI/25/001/2006). Financial support provided to Chari Nithya by Alagappa University in the form of Research Fellowship is thankfully acknowledged.


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Chari Nithya
    • 1
  • Mansur Farzana Begum
    • 1
  • Shunmugiah Karutha Pandian
    • 1
    Email author
  1. 1.Department of BiotechnologyAlagappa UniversityKaraikudiIndia

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