Applied Microbiology and Biotechnology

, Volume 87, Issue 6, pp 2037–2045 | Cite as

Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha

  • Yung-Hun Yang
  • Christopher J. Brigham
  • Charles F. Budde
  • Paolo Boccazzi
  • Laura B. Willis
  • Mohd Ali Hassan
  • Zainal Abidin Mohd Yusof
  • ChoKyun Rha
  • Anthony J. Sinskey
Biotechnological Products and Process Engineering

Abstract

We employed systematic mixture analysis to determine optimal levels of acetate, propionate, and butyrate for cell growth and polyhydroxyalkanoate (PHA) production by Ralstonia eutropha H16. Butyrate was the preferred acid for robust cell growth and high PHA production. The 3-hydroxyvalerate content in the resulting PHA depended on the proportion of propionate initially present in the growth medium. The proportion of acetate dramatically affected the final pH of the growth medium. A model was constructed using our data that predicts the effects of these acids, individually and in combination, on cell dry weight (CDW), PHA content (%CDW), PHA production, 3HV in the polymer, and final culture pH. Cell growth and PHA production improved approximately 1.5-fold over initial conditions when the proportion of butyrate was increased. Optimization of the phosphate buffer content in medium containing higher amounts of butyrate improved cell growth and PHA production more than 4-fold. The validated organic acid mixture analysis model can be used to optimize R. eutropha culture conditions, in order to meet targets for PHA production and/or polymer HV content. By modifying the growth medium made from treated industrial waste, such as palm oil mill effluent, more PHA can be produced.

Keywords

Polyhydroxyalkanoate Ralstonia eutropha Organic acid Mixture model 

Supplementary material

253_2010_2699_MOESM1_ESM.doc (44 kb)
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253_2010_2699_MOESM2_ESM.doc (66 kb)
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253_2010_2699_MOESM3_ESM.doc (29 kb)
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253_2010_2699_MOESM4_ESM.doc (62 kb)
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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yung-Hun Yang
    • 1
    • 2
  • Christopher J. Brigham
    • 1
  • Charles F. Budde
    • 3
  • Paolo Boccazzi
    • 1
  • Laura B. Willis
    • 1
    • 4
  • Mohd Ali Hassan
    • 5
  • Zainal Abidin Mohd Yusof
    • 6
  • ChoKyun Rha
    • 4
  • Anthony J. Sinskey
    • 1
    • 7
    • 8
  1. 1.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA
  2. 2.Department of Microbial EngineeringKonkuk UniversitySeoulRepublic of Korea
  3. 3.Department of Chemical EngineeringMassachusetts Institute of TechnologyCambridgeUSA
  4. 4.Biomaterials Science and Engineering LaboratoryMassachusetts Institute of TechnologyCambridgeUSA
  5. 5.Department of Biotechnology and Biomolecular ScienceUniversiti Putra MalaysiaSerdangMalaysia
  6. 6.Research & Technology DivisionSIRIM BerhadShah AlamMalaysia
  7. 7.Division of Health Sciences TechnologyMassachusetts Institute of TechnologyCambridgeUSA
  8. 8.Engineering Systems DivisionMassachusetts Institute of TechnologyCambridgeUSA

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