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Applied Microbiology and Biotechnology

, Volume 87, Issue 5, pp 1803–1811 | Cite as

Establishment of l-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering

  • Aloke Kumar Bera
  • Miroslav Sedlak
  • Aftab Khan
  • Nancy W. Y. HoEmail author
Applied Genetics and Molecular Biotechnology

Abstract

Cost-effective and efficient ethanol production from lignocellulosic materials requires the fermentation of all sugars recovered from such materials including glucose, xylose, mannose, galactose, and l-arabinose. Wild-type strains of Saccharomyces cerevisiae used in industrial ethanol production cannot ferment d-xylose and l-arabinose. Our genetically engineered recombinant S. cerevisiae yeast 424A(LNH-ST) has been made able to efficiently ferment xylose to ethanol, which was achieved by integrating multiple copies of three xylose-metabolizing genes. This study reports the efficient anaerobic fermentation of l-arabinose by the derivative of 424A(LNH-ST). The new strain was constructed by over-expression of two additional genes from fungi l-arabinose utilization pathways. The resulting new 424A(LNH-ST) strain exhibited production of ethanol from l-arabinose, and the yield was more than 40%. An efficient ethanol production, about 72.5% yield from five-sugar mixtures containing glucose, galactose, mannose, xylose, and arabinose was also achieved. This co-fermentation of five-sugar mixture is important and crucial for application in industrial economical ethanol production using lignocellulosic biomass as the feedstock.

Keywords

l-arabinose Pentose fermentation l-arabitol 4-dehydrogenase l-xylulose reductase Saccharomyces cerevisiae Ethanol 

Notes

Acknowledgement

The project was financially supported by the US Department of Energy Biomass Program, Contract G017059-16649.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Aloke Kumar Bera
    • 1
  • Miroslav Sedlak
    • 1
    • 2
  • Aftab Khan
    • 1
  • Nancy W. Y. Ho
    • 1
    • 3
    Email author
  1. 1.Laboratory of Renewable Resources EngineeringPurdue UniversityWest LafayetteUSA
  2. 2.Department of Agricultural and Biological EngineeringPurdue UniversityWest LafayetteUSA
  3. 3.School of Chemical EngineeringPurdue UniversityWest LafayetteUSA

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