Skip to main content

Advertisement

Springer Nature Link
Log in

Marine yeasts as biocontrol agents and producers of bio-products

  • Mini-Review
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

As some species of marine yeasts can colonize intestine of marine animals, they can be used as probiotics. It has been reported that β-glucans from marine yeast cells can be utilized as immuno-stimulants in marine animals. Some siderophores or killer toxins produced by marine yeasts have ability to inhibit growth of pathogenic bacteria or kill pathogenic yeasts in marine animals. The virulent factors from marine pathogens can be genetically displayed on marine yeast cells, and the yeast cells displaying the virulent factors can stimulate marine animals to produce specific antibody against the pathogens. Some marine yeast cells are rich in proteins and essential amino acids and can be used in nutrition for marine animals. The marine yeast cells rich in lipid can be used for biodiesel production. Recently, it has been reported that some strains of Yarrowia lipolytica isolated from marine environments can produce nanoparticles. Because many marine yeasts can remove organic pollutants and heavy metals, they can be applied to remediation of marine environments. It has been shown that the enzymes produced by some marine yeasts have many unique properties and many potential applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from $39.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  • Agnihotri M, Joshi S, Kumar AR, Zinjarde S, Kulkarni S (2009) Biosynthesis of gold nanoparticles by the tropical marine yeast Yarrowia lipolytica NCIM 3589. Mat Lett 63:1231–1234

    Article  CAS  Google Scholar 

  • Ahrean DG, Meyer SP, Standard PG (1971) The role of yeasts in the decomposition of oils in marine environments. In: Murray ED (ed) Developments in Industrial Microbiology, vol 12, American Institute of Biological Sciences. Washington, DC, pp 126–134

    Google Scholar 

  • Amaral PFF, da Silva JM, Lehocky M, Barros-Timmons MAMV, Coelho MAZ, Marrucho IM, Coutinho JAP (2006) Production and characterization of a bioemulsifier from Yarrowia lipolytica. Proc Biochem 41:1894–1898

    Article  CAS  Google Scholar 

  • Bankar AV, Kumar AR, Zinjarde SS (2009a) Environmental and industrial applications of Yarrowia lipolytica. Appl Microbiol Biotechnol 84:847–865

    Article  CAS  Google Scholar 

  • Bankar AV, Kumar AR, Zinjarde SS (2009b) Removal of Chromium (VI) ions from aqueous solution by adsorption onto two marine isolates of Yarrowia lipolytica. J Hazard Mater. doi:10.1016/j.jhazmat.2009.04.070

    Google Scholar 

  • Barbeyron T, Gerard A, Potin P, Henrissat B, Kloareg B (1998) The kappa-carrageenase of the marine bacterium Cytophaga drobachiensis. Structural and phylogenetic relationships within family-16 glycoside hydrolases. Mol Biol Evol 15:528–537

    CAS  Google Scholar 

  • Brown MR, Barrett SM, Volkman JK, Nearhos SP, Nell JA (1996) Biochemical composition of new yeasts and bacteria evaluated as food for bivalve aquaculture. Aquaculture 143:341–360

    Article  CAS  Google Scholar 

  • Chang CF, Su MS, Chen HY, Liao IC (2003) Dietary β-1, 3-glucan effectively improve immunity and survival of Penaeus monodon challenged with white spot syndrome virus. Fish Shellfish Immunol 15:297–310

    Article  CAS  Google Scholar 

  • Chi ZM, Yan KR, Gao LM, Li J, Wang XH, Wang L (2008a) Diversity of marine yeasts with high protein content and evaluation of their nutritive compositions. J Mar Biol Assoc UK 88:1–6

    Google Scholar 

  • Chi Z, Wang L, Ju L, Chi ZM (2008b) Optimization of riboflavin production by the marine yeast Candida membranifaciens subsp. flavinogenie W14-3 using response surface methodology. Ann Microbiol 58:677–681

    Article  CAS  Google Scholar 

  • Chi ZM, Chi Z, Zhang T, Yue L, Li J, Wang X (2009a) Production, characterization and gene cloning of the extracellular enzymes from the marine-derived yeasts and their potential applications. Biotechnol Adv 27:236–255

    Article  CAS  Google Scholar 

  • Chi ZM, Wang F, Chi Z, Yue LX, Liu GL, Zhang T (2009b) Bioproducts from Aureobasidium pullulans, a biotechnologically important yeast. Appl Microbiol Biotechnol 82:793–804

    Article  CAS  Google Scholar 

  • Chi ZM, Chi Z, Zhang T, Liu GL, Yue L (2009c) Inulinase expressing microorganisms and applications of inulinases. Appl Microbiol Biotechnol 82:211–220

    Article  CAS  Google Scholar 

  • Gao LM, Chi ZM, Sheng J, Ni XM (2007) Single-cell protein production from Jerusalem artichoke extract by a recently isolated marine yeast Cryptococcus aureus G7a and its nutritive analysis. Appl Microbiol Biotechnol 77:825–832

    Article  CAS  Google Scholar 

  • Gatesoupe FJ (2007) Live yeasts in the gut: natural occurrence, dietary introduction, and their effects on fish health and development. Aquaculture 267:20–30

    Article  Google Scholar 

  • Han F, Gong QH, Song K, Li JB, Yu WG (2004) Cloning, sequence analysis and expression of gene AlyVI encoding alginate lyase from marine bacterium Vibrio sp. QY101. DNA Sequence 0:1–8

    Google Scholar 

  • Hassan M, Blanc PJ, Granger LM, Pareilleux A, Goma G (1996) Influence of nitrogen and iron limitations on lipid production by Cryptococcus curvatus grown in batch and fed-batch culture. Proc Biochem 31:355–361

    Article  CAS  Google Scholar 

  • Hossain MB, Jalal MAF, van der Helm D (1998) Gallium-complex of anguibactin, a siderophore from fish pathogen Vibrio anguillarum. J Chem Cryst 28:57–60

    Article  CAS  Google Scholar 

  • Jain MR, Zinjarde SS, Deobagkar DD, Deobagkar DN (2004) 2, 4, 6-Trinitrotoluene transformation by a tropical marine yeast, Yarrowia lipolytica NCIM 3589. Mar Pollut Bull 49:783–788

    Article  CAS  Google Scholar 

  • Johnson L (2008) Iron and siderophores in fungal–host interactions. Mycol Resear 112:170–183

    Article  CAS  Google Scholar 

  • Klassen R, Meinhardt F (2005) Induction of DNA damage and apoptosis in Saccharomyces cerevisiae by a yeast killer toxin. Cell Microbiol 73:393–401

    Article  Google Scholar 

  • Kurtzman CP, Fell JW (2000) The yeasts: a taxonomic study, 4th revised and enlarged edition. Elsevier, Amsterdam, pp 1–525

    Google Scholar 

  • Lee AB, Park JH, Yoon SC, Kim JM, Kong IS (2000) Sequence analysis of a β-agarase gene (PjaA) from Pseudomonas sp. isolated from marine environment. J Biosci Bioengineer 89:485–488

    Article  CAS  Google Scholar 

  • Li J, Woo NYS (2003) Pathogenicity of Vibrios in fish: an overview. J Ocean Univ Qingdao 2:117–128

    Google Scholar 

  • Li C, Song L, Zhao J, Zhu L, Zou H, Zhang H, Wang H, Cai Z (2007) Preliminary study on a potential antibacterial peptide derived from histone H2A in hemocytes of scallop Chlamys farreri. Fish Shellfish Immunol 22:663–672

    Article  Google Scholar 

  • Li Q, Du W, Liu D (2008) Perspectives of microbial oils for biodiesel production. Appl Microbiol Biotechnol 80:749–756

    Article  CAS  Google Scholar 

  • Li M, Liu GL, Chi Z, Chi ZM (2010) Single cell oil production from hydrolysate of cassava starch by marine-derived yeast Rhodotorula mucilaginosa TJY15a. Biomass and Bioenergy 34:101–107

    Article  CAS  Google Scholar 

  • Liu G, Yue L, Chi Z, Yu W, Chi ZM, Madzak C (2009) The surface display of the alginate lyase on the cells of Yarrowia lipolytica for hydrolysis of alginate. Mar Biotechnol 11:619–626

    Article  CAS  Google Scholar 

  • Magliani W, Conti S, Gerloni M, Bertolotti D, Polonelli L (1997) Yeast killer systems. Clin Microbiol Rev 10:369–400

    CAS  Google Scholar 

  • Magliani W, Conti S, Travassos LR, Polonelli L (2008) From yeast killer toxins to antibiobodies and beyond. FEMS Microbiol Lett 288:1–8

    Article  CAS  Google Scholar 

  • Mao Z, Yu L, You Z, Wei Y, Liu Y (2007) Cloning, expression and immunogenicity analysis of five outer membrane proteins of Vibrio parahaemolyticus zj2003. Fish Shellfish Immunol 23:567–575

    Article  CAS  Google Scholar 

  • Marquina D, Santos A, Peinado JM (2002) Biology of killer yeasts. Int Microbiol 5:65–71

    Article  CAS  Google Scholar 

  • Martin K, McDougall BM, Jayus SM, Chen J, Seviour RJ (2007) Biochemistry and molecular biology of exocellular fungal β-(1, 3)- and β-(1, 6)-glucanases. FEMS Microbiol Rev 31:168–192

    Article  CAS  Google Scholar 

  • Meng X, Yang J, Xu X, Zhang L, Nie Q, Xian M (2009) Biodiesel production from oleaginous microorganisms. Renewable Energy 34:1–5

    Article  Google Scholar 

  • Moore MM, Strom MB (2003) Infection and mortality by the yeast Metschnikowia bicuspidata var. bicuspidata in Chinook salmon fed live adult brine shrimp (Artemia franciscana). Aquaculture 220:43–57

    Article  Google Scholar 

  • Oswal N, Sarma PM, Zinjarde SS, Pant A (2002) Palm oil mill effluent treatment by a tropical marine yeast. Biores Technol 85:35–37

    Article  CAS  Google Scholar 

  • Peng Y, Chi ZM, Wang XH, Li J (2009a) Purification and molecular characterization of exo-β-1, 3-glucanase from the marine-derived yeast Williopsis saturnus WC91-2. Appl Microbiol Biotechnol 85:85–94

    Article  CAS  Google Scholar 

  • Peng Y, Chi ZM, Wang XH, Li J (2009b) β-1, 3-glucanase inhibits activity of the killer toxin produced by the marine-derived yeast Williopsis saturnus WC91-2. Mar Biotechnol. doi:10.1007/s10126-009-9243-9

    Google Scholar 

  • Pimprikara PS, Joshi SS, Kumara AR, Zinjardea SS, Kulkarnib SK (2009) Influence of biomass and gold salt concentration on nanoparticle synthesis by the tropical marine yeast Yarrowia lipolytica NCIM 3589. Colloids Surf B: Biointerfaces 74:309–316

    Article  Google Scholar 

  • Ratledge C (2004) Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie 86:807–815

    Article  CAS  Google Scholar 

  • Ravindra AP (2000) Value-added food: single cell protein. Biotechnol Adv 18:459–479

    Article  Google Scholar 

  • Renshaw JC, Robson GD, Trinci APJ, Wiebe MG, Livens FR, Colllison D, Taylor RJ (2002) Fungal siderophores: structures, functions and applications. Mycol Res 106:1123–1142

    Article  CAS  Google Scholar 

  • Rhishipal R, Philip R (1998) Selection of marine yeasts for the generation of single cell protein from prawn-shell. Bioresour Technol 65:255–266

    Article  CAS  Google Scholar 

  • Riquelme M (1996) Fungal siderophores in plant–microbe interactions. Microbiol SEM 12:537–546

    CAS  Google Scholar 

  • Sajeevan TP, Philip R, Singh ISB (2006) Immunostimulatory effect of a marine yeast Candida sake S165 in Fenneropenaeus indicus. Aquaculture 257:150–155

    Article  Google Scholar 

  • Sheng J, Chi ZM, Li J, Gao LM, Gong F (2007) Inulinase production by the marine yeast Cryptococcus aureus G7a and inulin hydrolysis by the crude inulinase. Proc Biochem 42:805–811

    Article  CAS  Google Scholar 

  • Song YL, Liu JJ, Chan LC, Sung HH (1997) Glucan induced disease resistance in tiger shrimp (Penaeus monodon). Fish Vaccinol Dev Biol Stand 90:413–421

    CAS  Google Scholar 

  • Stahmann P, Revuelta JL, Seulberge H (2000) Three biotechnical processes Ashbya gossypii, Candida famata or Bacillus subtilis compete with using chemical riboflavin production. Appl Microbiol Biotechnol 53:509–516

    Article  CAS  Google Scholar 

  • Stefanidi E, Vorgias CE (2008) Molecular analysis of the gene encoding a new chitinase from the marine psychrophilic bacterium Moritella marina and biochemical characterization of the recombinant enzyme. Extremophiles 12:541–552

    Article  CAS  Google Scholar 

  • Strouhal M, Kizek R, Vacek J, Trnkova L, Nemec M (2003) Electrochemical study of heavy metals and metallothionein in yeast Yarrowia lipolytica. Bioelectronics 60:29–36

    CAS  Google Scholar 

  • Sun YH, Sun QH (1998) Studies on pathogenic organism and prevention and cure for explosive epidemic disease of parent prawn of Macrobrachium rosenbergh. J Fish China 22:56–60

    Google Scholar 

  • Sung HH, Kou GH, Song YI (1994) Vibriosis resistance induced by glucan treatment in tiger shrimp (Penaeus monodon). Fish Pathol 29:11–17

    CAS  Google Scholar 

  • Tovar-Ramírez D, Zambonino J, Cahu C, Gatesoupe FJ, Vázquez-Juárez R, Lésel R (2002) Effect of live yeast incorporation in compound diet on digestive enzyme activity in sea bass (Dicentrarchus labrax) larvae. Aquaculture 204:113–123

    Article  Google Scholar 

  • Ueda M, Tanaka A (2000) Genetic immobilization of proteins on the yeast cell surface. Biotechnol Adv 18:121–140

    Article  CAS  Google Scholar 

  • Verschuere L, Rombaut G, Sorgeloos P (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiol Mole Biol Rew 64:655–671

    Article  CAS  Google Scholar 

  • Wang X, Du ZJ, Chen JX, Li Y, Ji WS, Xu HS (2002) Use of probiotics in aquaculture. High Technol Lett 8:22–27

    Google Scholar 

  • Wang X, Chi ZM, Yue L, Li J, Li M, Wu L (2007a) A marine killer yeast against the pathogenic yeast strain in crab (Portunus trituberculatus) and an optimization of the toxin production. Microbiol Res 162:77–85

    Article  CAS  Google Scholar 

  • Wang X, Chi ZM, Yue L, Li J (2007b) Purification and characterization of killer toxin from a marine yeast Pichia anomala YF07b against the pathogenic yeast in crab. Curr Microbiol 55:396–401

    Article  CAS  Google Scholar 

  • Wang XH, Chi ZM, Li J (2007c) Isolation and identification of a marine killer yeast strain YF07b and cloning of gene encoding the killer toxin from the yeast. Acta Oceanologica Sinica 26:101–109

    CAS  Google Scholar 

  • Wang L, Yue L, Chi ZM, Wang X (2008a) Marine killer yeasts active against a yeast strain pathogenic to crab Portunus trituberculatus. Dis Aquat Organ 80:211–8

    Article  CAS  Google Scholar 

  • Wang L, Chi ZM, Wang X, Ju L, Chi Z, Guo N (2008b) Isolation and characterization of Candida membranifaciens subsp. flavinogenie W14-3, a novel riboflavin-producing marine yeast. Microbiol Res 163:255–266

    Article  CAS  Google Scholar 

  • Wang F, Yue L, Wang L, Madzak C, Li J, Wang X, Chi ZM (2009a) Genetic modification of the marine-derived yeast Yarrowia lipolytica with high-protein content using a GPI-anchor-fusion expression system. Biotechnol Prog 25:1297–1303

    Article  Google Scholar 

  • Wang W, Chi ZM, Chi Z, Li J, Wang XH (2009b) Siderophore production by the marine-derived Aureobasidium pullulans and its antimicrobial activity. Biores Technol 100:2639–2641

    Article  CAS  Google Scholar 

  • Wang W, Chi Z, Liu G, Buzdar MA, Chi ZM, Gu Q (2009c) Chemical and biological characterization of siderophore produced by the marine-derived Aureobasidium pullulans HN6.2 and its antibacterial activity. Biometals 22:965–972

    Article  CAS  Google Scholar 

  • Xu WJ, Xu HX, Jin HW (2003) Studies on milky disease of Portunus trituberculatus. J Oceanol Zhejiang Province 3:209–213

    Google Scholar 

  • Zhang FL, Chen JX, Chi ZM, Wu LF (2006) Expression and processing of Vibrio anguillarum zinc-metalloprotease in Escherichia coli. Arch Microbiol 186:11–20

    Article  CAS  Google Scholar 

  • Zhang T, Gong F, Chi Z, Liu G, Chi ZM, Sheng J, Li J, Wang X (2009a) Cloning and characterization of the inulinase gene from a marine yeast Pichia guilliermondii and its expression in Pichia pastoris. Antonie van Leeuwenhoek 95:13–22

    Article  CAS  Google Scholar 

  • Zhang T, Chi ZM, Sheng J (2009b) A highly thermosensitive and permeable mutant of the marine yeast Cryptococcus aureus G7a potentially useful for single-cell protein production and its nutritive components. Mar Biotechnol 95:13–22

    CAS  Google Scholar 

  • Zhang T, Gong F, Peng Y, Chi ZM (2009c) Optimization for high-level expression of the Pichia guilliermondii recombinant inulinase in Pichia pastoris and characterization of the recombinant inulinase. Proc Biochem 44:1335–1339

    Article  CAS  Google Scholar 

  • Zhao CH, Zhang T, Chi ZM, Chi Z, Li J, Wang XH (2009) Single cell protein production from yacon extract using a highly thermosensitive and permeable mutant of the marine yeast Cryptococcus aureus G7a and its nutritive analysis. Bioproc Biosyst Eng. doi:10.1007/s00449-009-0376-z

    Google Scholar 

  • Zhu K, Chi ZM, Li J, Zhang F, Li M, Yasoda HN (2006) The surface display of haemolysin from Vibrio harveyi on yeast cells and their potential applications as live vaccine in marine fish. Vaccine 24:6046–6052

    Article  CAS  Google Scholar 

  • Zinjarde SS, Pant AA (2002) Hydrocarbon degraders from tropical marine environments. Mar Poll Bull 44:118–121

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the grant (No 30771645) from National Natural Science Foundation of China.

Author information

Authors and Affiliations

  1. Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China

    Zhen-Ming Chi, Guanglei Liu, Shoufeng Zhao, Jing Li & Ying Peng

Authors
  1. Zhen-Ming Chi
    View author publications

    Search author on:PubMed Google Scholar

  2. Guanglei Liu
    View author publications

    Search author on:PubMed Google Scholar

  3. Shoufeng Zhao
    View author publications

    Search author on:PubMed Google Scholar

  4. Jing Li
    View author publications

    Search author on:PubMed Google Scholar

  5. Ying Peng
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Zhen-Ming Chi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chi, ZM., Liu, G., Zhao, S. et al. Marine yeasts as biocontrol agents and producers of bio-products. Appl Microbiol Biotechnol 86, 1227–1241 (2010). https://doi.org/10.1007/s00253-010-2483-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-010-2483-9

Keywords