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Fermentation and metabolic characteristics of Gluconacetobacter oboediens for different carbon sources

Applied Microbiology and Biotechnology volume 87, pages 127–136 (2010)Cite this article

Abstract

The metabolism of Gluconacetobacter oboediens was investigated in relation to different carbon sources for the continuous cultures at the dilution rate of 0.05 h−1. The 13C-flux result implies the formation of metabolic recycles for the case of using glucose and acetate as carbon sources. When glucose and ethanol were used as carbon sources, the specific ethanol uptake rate and the specific acetate production rate increased as the feed ethanol concentration was increased from 40 to 60 g/l, while the specific CO2 production rate and the biomass concentration decreased, where the 13C-metabolic flux result indicates that the glycolysis, oxidative PP pathway, and the tricarboxylic acid (TCA) cycle were less active, resulting in less biomass concentration. The flux result also implies that oxaloacetate decarboxylase flux became negative, so that oxaloacetate is backed up by this pathway, resulting in less activity of glyoxylate pathway. When gluconate was added for the case of using glucose and ethanol as carbon sources, the acetate and cell concentrations as well as gluconate concentrations increased. The glucose and ethanol concentrations decreased concomitantly with the increased feed gluconate concentration. In accordance with these fermentation characteristics, the enzyme activity result indicates that glucose dehydrogenase and glucose-6-phosphate dehydrogenase pathways became less active, while the glycolysis and the TCA cycle was activated as the feed gluconate concentration was increased.

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Abbreviations

G6P:

Glucose-6-phosphate

F6P:

Fructose-6-phosphate

GAP:

Glyceraldehyde-3-phosphate

3PG:

3-Phosphoglycerate

PEP:

Phosphoenol pyruvate

PYR:

Pyruvate

GLCN:

Gluconate

6PG:

6-Phosphogluconate

PL5P:

Ribulose-5-phosphate

R5P:

Ribose-5-phosphate

X5P:

Xylulose-5-phosphate

S7P:

Sedoheptulose-7-phosphate

E4P:

Erythrose-4-phosphate

2KD6PG:

2-keto-3-deoxy-6-phosphogluconate

AcCoA:

Acetyl-coenzyme A

AC:

Acetate

ICIT:

Isocitrate

AKG:

α-keto-gluterate

SUCC:

Succinate

FUM:

Fumarate

MAL:

Malate

OAA:

Oxaloacetate

GLX:

Glyoxylate

PTS:

Phosphoglucosetransferase system

Pgi:

Phosphoglucose isomerase

Pfk:

Phosphofructo kinase

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

Eno:

Enolase

Pyk:

Pyruvate kinase

G6PDH:

Glucose-6-phosphate dehydrogenase

6PGDH:

6-Phosphogluconate dehydrogenase

PDH:

Pyruvate dehydrogenase complex

OAADC:

Oxaloacetate decarboxylase

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Acknowledgement

Dr. Koichi Kondo of Mizkan Group Corporation, who made important comments on the manuscript, is acknowledged.

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Affiliations

  1. Department of Bioscience & Bioinformatics, Kyushu Institute of Technology, 680-4, Kawazu, Iizuka, Fukuoka, 820-8502, Japan

    Dayanidhi Sarkar, Yuta Hasebe, Pei Yee Ho & Kazuyuki Shimizu

  2. Mizkan Group Corporation, Tokyo, Japan

    Masahiro Yabusaki, Shuji Kohmoto & Takayuki Kaga

  3. Institute for Advanced Biosciences, Keio University, Tsuroka, Yamagata, 997-0017, Japan

    Kazuyuki Shimizu

Authors
  1. Dayanidhi Sarkar
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  2. Masahiro Yabusaki
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  3. Yuta Hasebe
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  4. Pei Yee Ho
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  5. Shuji Kohmoto
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  6. Takayuki Kaga
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  7. Kazuyuki Shimizu
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Correspondence to Kazuyuki Shimizu.

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Sarkar, D., Yabusaki, M., Hasebe, Y. et al. Fermentation and metabolic characteristics of Gluconacetobacter oboediens for different carbon sources. Appl Microbiol Biotechnol 87, 127–136 (2010). https://doi.org/10.1007/s00253-010-2474-x

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  • DOI: https://doi.org/10.1007/s00253-010-2474-x

Keywords

  • Gluconacetobacter oboediens
  • Chemostat culture
  • Enzyme activity
  • 13C-labeling experiment
  • Metabolic flux analysis
  • Acetate fermentation