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Gene transcription profiling of Fusarium graminearum treated with an azole fungicide tebuconazole

Applied Microbiology and Biotechnology volume 85, pages 1105–1114 (2010)Cite this article

Abstract

Using a deep serial analysis of gene expression (DeepSAGE) sequencing approach, we profiled the transcriptional response of Fusarium graminearum to tebuconazole, a most widely used azole fungicide. By comparing the expression of genes in F. graminearum treated and untreated with tebuconazole, we identified 324 and 155 genes showing more than a 5-fold increase and decrease, respectively, in expression upon tebuconazole treatment. These genes are involved in a variety of cell functions including egrosterol biosynthesis, transcription, and cellular metabolism. The validity of DeepSAGE results were confirmed by real-time PCR analysis of expression of 20 genes with different expression levels in the DeepSAGE analysis. The results from this study provide useful information in understanding the mechanisms for the responses of F. graminearum to azole fungicides.

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Acknowledgments

The research was supported by the Special Program for Agricultural Research (nyhyzx07-048), the 863 program (2007AA10Z422), and the grant MATS from Department of Agriculture to Z. Ma.

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Affiliations

  1. Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 268 Kaixuan Road, Hangzhou, 310029, China

    Xin Liu, Jinhua Jiang, Yanni Yin & Zhonghua Ma

  2. Zhejiang-California International Nanosystems Institute, 268 Kaixuan Road, Hangzhou, 310029, China

    Jiaofang Shao

Authors
  1. Xin Liu
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  2. Jinhua Jiang
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  3. Jiaofang Shao
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  4. Yanni Yin
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  5. Zhonghua Ma
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Corresponding author

Correspondence to Zhonghua Ma.

Electronic supplementary materials

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Supplementary Table 1

A list of genes up-regulated in expression by more than 5 folds in F. graminearum treated with tebuconazole at 2.5 μg/ml for six hours (DOC 57 kb)

Supplementary Table 2

A list of genes down-regulated in expression by more than 5 folds in F. graminearum treated with tebuconazole at 2.5 μg/ml for six hours (DOC 41 kb)

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Liu, X., Jiang, J., Shao, J. et al. Gene transcription profiling of Fusarium graminearum treated with an azole fungicide tebuconazole. Appl Microbiol Biotechnol 85, 1105–1114 (2010). https://doi.org/10.1007/s00253-009-2273-4

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  • DOI: https://doi.org/10.1007/s00253-009-2273-4

Keywords

  • DeepSAGE
  • Fusarium graminearum
  • Real-time PCR
  • Tebuconazole
  • Transcriptome