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DyP-like peroxidases of the jelly fungus Auricularia auricula-judae oxidize nonphenolic lignin model compounds and high-redox potential dyes

Abstract

The jelly fungus Auricularia auricula-judae produced an enzyme with manganese-independent peroxidase activity during growth on beech wood (∼300 U l−1). The same enzymatic activity was detected and produced at larger scale in agitated cultures comprising of liquid, plant-based media (e.g. tomato juice suspensions) at levels up to 8,000 U l−1. Two pure peroxidase forms (A. auricula-judae peroxidase (AjP I and AjP II) could be obtained from respective culture liquids by three chromatographic steps. Spectroscopic and electrophoretic analyses of the purified proteins revealed their heme and peroxidase nature. The N-terminal amino acid sequence of AjP matched well with sequences of fungal enzymes known as “dye-decolorizing peroxidases”. Homology was found to the N-termini of peroxidases from Marasmius scorodonius (up to 86%), Thanatephorus cucumeris (60%), and Termitomyces albuminosus (60%). Both enzyme forms catalyzed not only the conversion of typical peroxidase substrates such as 2,6-dimethoxyphenol and 2,2′-azino-bis(3-ethylthiazoline-6-sulfonate) but also the decolorization of the high-redox potential dyes Reactive Blue 5 and Reactive Black 5, whereas manganese(II) ions (Mn2+) were not oxidized. Most remarkable, however, is the finding that both AjPs oxidized nonphenolic lignin model compounds (veratryl alcohol; adlerol, a nonphenolic β-O-4 lignin model dimer) at low pH (maximum activity at pH 1.4), which indicates a certain ligninolytic activity of dye-decolorizing peroxidases.

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Acknowledgment

We thank Martin Kluge (Inge), Matthias Kinne (Konrad), and Elisabet Aranda for their useful comments and discussions as well as Ulrike Schneider and Monika Brandt for their technical assistance. The work was supported by the European Union (integrated project “Biorenew”) and the German Environmental Foundation (DBU, project 13225-32).

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Correspondence to Christiane Liers.

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Liers, C., Bobeth, C., Pecyna, M. et al. DyP-like peroxidases of the jelly fungus Auricularia auricula-judae oxidize nonphenolic lignin model compounds and high-redox potential dyes. Appl Microbiol Biotechnol 85, 1869–1879 (2010). https://doi.org/10.1007/s00253-009-2173-7

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  • DOI: https://doi.org/10.1007/s00253-009-2173-7

Keywords

  • Dye-decolorizing peroxidases
  • Jelly fungi
  • White rot
  • Nonphenolic β-O-4 lignin model compound
  • Azo and anthraquinone dyes