Abstract
The separation of structurally related impurities from pharmaceutical plasmid DNA by highly scalable purification techniques is a challenge for biochemical engineering. Next to RNA, proteins, and lipopolysaccharides, the chromosomal DNA of the plasmid replicating host has to be removed. Here, we describe the application of reverse micellar extraction for the separation of chromosomal from plasmid DNA. By applying different procedures for alkaline lysis, bacterial lysates with different amounts of chromosomal DNA were generated. A reverse micellar extraction step enabled us to deplete the concentration of this impurity below the required level of 50 mg g−1 of plasmid DNA with almost complete plasmid recovery.
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Acknowledgments
The research project was funded by the German Research Foundation (Deutsche Forschungsgemeinschaft) and the Max-Buchner-Forschungsstiftung. Their support is gratefully acknowledged.
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Tschapalda, K., Streitner, N., Voß, C. et al. Generation of chromosomal DNA during alkaline lysis and removal by reverse micellar extraction. Appl Microbiol Biotechnol 84, 199–204 (2009). https://doi.org/10.1007/s00253-009-2088-3
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DOI: https://doi.org/10.1007/s00253-009-2088-3