Abstract
This study evaluates the potential of bio-augmentation to improve the degradation of recalcitrant nonylphenol during the wastewater treatment in membrane bioreactors (MBR). One MBR containing activated sludge was bio-augmented using multistep inoculation with freeze dried Sphingomonas sp. strain TTNP3, whereas a second control reactor contained activated sludge solely. The 14C-labeled-nonylphenol isomer (4-[1-ethyl-1,3-dimethylpentyl]phenol) was applied as a single pulse. Bio-augmentation resulted in an immediate increase of dissolved radioactivity in the effluent in comparison to the control reactor (13% and 2% of initially applied radioactivity after 1 day, respectively). After 5 days of operation, the retentate of the bio-augmented reactor contained only 7% of the initial radioactivity in contrast to 50% in the control reactor. The radioactivity associated to the mixed liquor suspended solids, i.e., the suspension of biomass and other solids on the retentate side of the membrane, was mainly found as non-extractable residues that were increasingly formed during prolonged reactor operation, especially for the control MBR. HPLC-LSC and GC-MSn analyses revealed that the bio-augmented reactor produced more polar hydroquinone as main degradation intermediate, whereas the control reactor effluent contained a complex mixture of apolar compounds with shortened oxidized alkyl chains. Thus, the apparent differences in the behavior of nonylphenol between the reactors were due to the catabolism of nonylphenol conferred by bio-augmentation with Sphingomonas sp. strain TTNP3.
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Boon N, De Gelder L, Lievens H, Siciliano SD, Top EM, Verstraete W (2002) Bio-augmenting bioreactors for the continuous removal of 3-Chloroaniline by a slow release approach. Environ Sci Technol 36:4698–4704
Cirja M, Zühlke S, Ivashechkin P, Schäffer A, Corvini PFX (2006) Fate of a 14C-labeled nonylphenol isomer in a laboratory-scale membrane bioreactor. Environ Sci Technol 40:6131–6136
Cirja M, Ivashechkin P, Schäffer A, Corvini PFX (2008) Factors affecting the removal of organic micropollutants from wastewater in conventional treatment plants (CTP) and membrane bioreactors (MBR). Rev Environ Sci Biotechnol 7:61–78
Corvini PFX, Meesters RJW, Schäffer A, Schröder HF, Vinken R, Hollender J (2004a) Degradation of a nonylphenol single isomer by Sphingomonas sp. strain TTNP3 leads to a hydroxylation-induced migration product. Appl Environ Microbiol 70:6897–6900
Corvini PFX, Vinken R, Hommes G, Schmidt B, Dohmann M (2004b) Degradation of the radioactive and non-labelled branched 4(3′, 5′-dimethyl 3′-heptyl)-phenol nonylphenol isomer by Sphingomonas TTNP3. Biodegradation 15:9–18
Corvini PFX, Hollender J, Ji R, Schumacher S, Prell J, Hommes G, Priefer U, Vinken R, Schäffer A (2006a) The degradation of α-quaternary nonylphenol isomers by Sphingomonas sp. strain TTNP3 involves a type II ipso-substitution mechanism. Appl Microbiol Biotechnol 70:114–122
Corvini PFX, Schäffer A, Schlosser D (2006b) Microbial degradation of nonylphenol and other alkylphenols - Our evolving view. Appl Microbiol Biotechnol 72:223–243
De Wildeman S, Linthout G, Van Langenhove H, Verstraete W (2004) Complete lab-scale detoxification of groundwater containing 1, 2-dichloroethane. Appl Microbiol Biotechnol 63:609–612
Dhouib A, Ellouz M, Aloui F, Sayadi S (2006) Effect of bio-augmentation of activated sludge with white-rot fungi on olive mill wastewater detoxification. Lett Appl Microbiol 42:405–411
El Fantroussi S, Agathos SN (2005) Is bio-augmentation a feasible strategy for pollutant removal and site remediation? Curr Opin Microbiol 8:268–275
Fialho AM, Moreira LM, Granja AT, Popescu AO, Hoffmann K, Sá-Correia I (2008) Occurrence, production, and applications of gellan: Current state and perspectives. Appl Microbiol Biotechnol 79:889–900
Fujii K, Yamamoto R, Tanaka T, Hirakawa T, Kikuchi S (2003) Potential of a new biotreatment: Sphingomonas cloacae S-3 T degrades nonylphenol in industrial wastewater. J Ind Microbiol Biotechnol 30:531–535
Gevao B, Jones KC, Semple KT (2005) Formation and release of non-extractable 14C-Dicamba residues in soil under sterile and non-sterile regimes. Environ Pollut 133:17–24
Hailei W, Guosheng L, Ping L, Feng P (2006) The effect of bio-augmentation on the performance of sequencing batch reactor and sludge characteristics in the treatment process of papermaking wastewater. Bioprocess Biosyst Eng 29:283–289
Johnsen AR, Karlson U (2004) Evaluation of bacterial strategies to promote the bioavailability of polycyclic aromatic hydrocarbons. Appl Microbiol Biotechnol 63:452–459
Kolvenbach B, Schlaich N, Raoui Z, Prell J, Zühlke S, Schäffer A, Guengerich FP, Corvini PFX (2007) Degradation pathway of bisphenol A: Does ipso substitution apply to phenols containing a quaternary α-carbon structure in the para position? Appl Environ Microbiol 73:4776–4784
Kouloumbos VN, Schäffer A, Corvini PFX (2008a) Impact of sewage sludge conditioning and dewatering on the fate of nonylphenol in sludge-amended soils. Water Res 42:3941–3951
Kouloumbos VN, Schäffer A, Corvini PFX (2008b) The role of sludge conditioning and dewatering in the fate of nonylphenol in sludge-amended soils. Water Sci Technol 57:329–335
Li C, Ji R, Vinken R, Hommes G, Bertmer M, Schäffer A, Corvini PFX (2007) Role of dissolved humic acids in the biodegradation of a single isomer of nonylphenol by Sphingomonas sp. Chemosphere 68:2172–2180
Loperena L, Saravia V, Murro D, Ferrari MD, Lareo C (2006) Kinetic properties of a commercial and a native inoculum for aerobic milk fat degradation. Bioresour Technol 97:2160–2165
McLaughlin H, Farrell A, Quilty B (2006) Bio-augmentation of activated sludge with two Pseudomonas putida strains for the degradation of 4-chlorophenol. J Environ Sci Health Part A Toxic/Hazard Subst Environ Eng 41:763–777
Olaniran AO, Pillay D, Pillay B (2006) Biostimulation and bio-augmentation enhances aerobic biodegradation of dichloroethenes. Chemosphere 63:600–608
Preuss TG, Gehrhardt J, Schirmer K, Coors A, Rubach M, Russ A, Jones PD, Giesy JP, Ratte HT (2006) Nonylphenol isomers differ in estrogenic activity. Environ Sci Technol 40:5147–5153
Rogers HR (1996) Sources, behaviour and fate of organic contaminants during sewage treatment and in sewage sludges. Sci Total Environ 185:3–26
Soares A, Guieysse B, Jefferson B, Cartmell E, Lester JN (2008) Nonylphenol in the environment: A critical review on occurrence, fate, toxicity and treatment in wastewaters. Environ Int 34:1033–1049
Soares A, Murto M, Guieysse B, Mattiasson B (2006) Biodegradation of nonylphenol in a continuous bioreactor at low temperatures and effects on the microbial population. Appl Microbiol Biotechnol 69:597–606
Vinken R, Schmidt B, Schäffer A (2002) Synthesis of tertiary 14C-labelled nonylphenol isomers. J Label Compd Radiopharm 45:1253–1263
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We thank the European Commission for funding AQUAbase under the Human Resources and Mobility Activity within the sixth Framework Program under contract number MEST-CT-2004-505169.
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Cirja, M., Hommes, G., Ivashechkin, P. et al. Impact of bio-augmentation with Sphingomonas sp. strain TTNP3 in membrane bioreactors degrading nonylphenol. Appl Microbiol Biotechnol 84, 183–189 (2009). https://doi.org/10.1007/s00253-009-2050-4
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DOI: https://doi.org/10.1007/s00253-009-2050-4