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Bioproduction of lauryl lactone and 4-vinyl guaiacol as value-added chemicals in two-phase biotransformation systems

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Abstract

Recombinant Escherichia coli whole-cell biocatalysts harboring either a Baeyer–Villiger monooxygenase or ferulic acid decarboxylase were employed in organic-aqueous two-phase bioreactor systems. The feasibility of the bioproduction of water-insoluble products, viz., lauryl lactone from cyclododecanone and 4-vinyl guaiacol from ferulic acid were examined. Using hexadecane as the organic phase, 10∼16 g of lauryl lactone were produced in a 3-l bioreactor that operated in a semicontinuous mode compared to 2.4 g of product in a batch mode. For the decarboxylation of ferulic acid, a new recombinant biocatalyst, ferulic acid decarboxylase derived from Bacillus pumilus, was constructed. Selected solvents as well as other parameters for in situ recovery of vinyl guaiacol were investigated. Up to 13.8 g vinyl guaiacol (purity of 98.4%) were obtained from 25 g of ferulic acid in a 2-l working volume bioreactor by using octane as organic phase. These selected examples highlight the superiority of the two-phase biotransformations systems over the conventional batch mode.

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Acknowledgements

We thank the Climate Change Technology and Innovation Biotechnology Program of the Canadian Biomass Innovation Network of Natural Resources Canada for financial support. We are grateful to H. Leisch for suggestions and help with the manuscript.

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  1. Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, QC, H4P 2R2, Canada

    Jianzhong Yang, Shaozhao Wang, Marie-Josée Lorrain, Denis Rho, Kofi Abokitse & Peter C. K. Lau

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  1. Jianzhong Yang
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  2. Shaozhao Wang
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  3. Marie-Josée Lorrain
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Correspondence to Peter C. K. Lau.

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Yang, J., Wang, S., Lorrain, MJ. et al. Bioproduction of lauryl lactone and 4-vinyl guaiacol as value-added chemicals in two-phase biotransformation systems. Appl Microbiol Biotechnol 84, 867–876 (2009). https://doi.org/10.1007/s00253-009-2026-4

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  • DOI: https://doi.org/10.1007/s00253-009-2026-4

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