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Heterologous expression of a Tpo1 homolog from Arabidopsis thaliana confers resistance to the herbicide 2,4-D and other chemical stresses in yeast

  • Applied Genetics and Molecular Biotechnology
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Abstract

The understanding of the molecular mechanisms underlying acquired herbicide resistance is crucial in dealing with the emergence of resistant weeds. Saccharomyces cerevisiae has been used as a model system to gain insights into the mechanisms underlying resistance to the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). The TPO1 gene, encoding a multidrug resistance (MDR) plasma membrane transporter of the major facilitator superfamily (MFS), was previously found to confer resistance to 2,4-D in yeast and to be transcriptionally activated in response to the herbicide. In this work, we demonstrate that Tpo1p is required to reduce the intracellular concentration of 2,4-D. ScTpo1p homologs encoding putative plasma membrane MFS transporters from the plant model Arabidopsis thaliana were analyzed for a possible role in 2,4-D resistance. At5g13750 was chosen for further analysis, as its transcript levels were found to increase in 2,4-D stressed plants. The functional heterologous expression of this plant open reading frame in yeast was found to confer increased resistance to the herbicide in Δtpo1 and wild-type cells, through the reduction of the intracellular concentration of 2,4-D. Heterologous expression of At5g13750 in yeast also leads to increased resistance to indole-3-acetic acid (IAA), Al3+ and Tl3+. At5g13750 is the first plant putative MFS transporter to be suggested as possibly involved in MDR.

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Acknowledgments

We thank Dr. Estelle Remy for critical comments on the manuscript. This work was financially supported by FEDER and “Fundação para a Ciência e a Tecnologia” (FCT) (Contract PTDC/AGR-AAM/67858/2006 and PhD Fellowship SFRH/BD/40885/2007 to TRC).

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Authors and Affiliations

  1. Institute for Biotechnology and BioEngineering (IBB), Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisbon, Portugal

    Tânia R. Cabrito, Miguel C. Teixeira & Isabel Sá-Correia

  2. Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156, Oeiras, Portugal

    Alexandra A. Duarte & Paula Duque

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  1. Tânia R. Cabrito
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  2. Miguel C. Teixeira
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  3. Alexandra A. Duarte
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  4. Paula Duque
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  5. Isabel Sá-Correia
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Correspondence to Isabel Sá-Correia.

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Cabrito, T.R., Teixeira, M.C., Duarte, A.A. et al. Heterologous expression of a Tpo1 homolog from Arabidopsis thaliana confers resistance to the herbicide 2,4-D and other chemical stresses in yeast. Appl Microbiol Biotechnol 84, 927–936 (2009). https://doi.org/10.1007/s00253-009-2025-5

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  • DOI: https://doi.org/10.1007/s00253-009-2025-5

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