Skip to main content
SpringerLink
Log in

Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet

  • Applied microbial and cell physiology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The objective of this study was to evaluate the effects of Lactobacillus plantarum MA2, an isolate from Chinese traditional Tibet kefir, on cholesterol-lowering and microflora of rat in vivo. Rats were fed on cholesterol-enriched experimental diet, supplemented with lyophilized L. plantarum MA2 powder, with a dose of 1011 cells/day per mice. The results showed that L. plantarum MA2 feeding significantly lowered serum total cholesterol, low-density lipoprotein cholesterol, and triglycerides level, while there was no change in high-density lipoprotein cholesterol. In addition, liver total cholesterol and triglycerides was also decreased. However, fecal cholesterol and triglycerides was increased significantly (P < 0.05) in comparison with the control. Also, L. plantarum MA2 increased the population of lactic acid bacteria and bifidobacteria in the fecal, but it did not change the number of Escherichia coli as compared to control. Moreover, pH, moisture, and organic acids in the fecal were also measured. The present results indicate the probiotic potential of the L. plantarum MA2 strain in hypocholesterolemic effect and also increasing the probiotic count in the intestine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Agerbaeck M, Gerdes LU, Richelsen B (1995) Hypocholesterolemic effect of a new fermented milk product in healthy middle-aged men. Eur J Clin Nutr 49:346–352

    Google Scholar 

  • Akalin AS, Gonc S, Duzel S (1997) Influence of yogurt and acidophilus yogurt on serum cholesterol levels in mice. J Dairy Sci 80:2721–2725

    CAS  PubMed  Google Scholar 

  • Bazzare TL, Wu SM, Yuhas JA (1983) Total and HDL cholesterol concentrations following yogurt and calcium supplementation. Nutr Rep Int 28:1225–1232

    Google Scholar 

  • Beena A, Prasad V (1997) Effect of yogurt and bifidus yogurt fortified with skim milk powder, condensed whey and lactose-hydrolyzed condensed whey on serum cholesterol and triacylgtycerol levels in rats. J Dairy Res 64:453–457

    CAS  PubMed  Google Scholar 

  • Bernardeau M, Vernoux JP, Gueguen M (2002) Safety and efficacy of probiotic lactobacilli in promoting growth in post-weaning Swiss mice. Int J Food Microbiol 77:19–27

    PubMed  Google Scholar 

  • Bernet MF, Brassart D, Neesar JR, Servin AL (1994) Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. Gut 35:483–489

    CAS  PubMed  PubMed Central  Google Scholar 

  • Chikai T, Nakao H, Uchida K (1987) Deconjugation of bile acids by human intestinal bacteria implanted in germ free rats. Lipids 22:669–671

    CAS  PubMed  Google Scholar 

  • Chiu CH, Lu TY, Tseng YY, Pan TM (2006) The effects of Lactobacillus-fermented milk on lipid metabolism in hamsters fed on high cholesterol diet. Appl Microbiol Biotechnol 71:238–245

    CAS  PubMed  Google Scholar 

  • Corzo G, Gilliland SE (1999) Bile salt hydrolase activity of three strains of Lactobacillus acidophilus. J Dairy Sci 82:472–480

    CAS  PubMed  Google Scholar 

  • Danielson AD, Peo ERJ, Shahani KM, Lewis AJ, Whalen PJ, Amer MA (1989) Anticholesterolemic property of Lactobacillus acidophilus yoghurt fed to mature boars. J Anim Sci 67:966–974

    CAS  PubMed  Google Scholar 

  • Delzenne NM, Kok N (2001) Effects of fructans-type prebiotics on lipid metabolism. Am J Clin Nutr 73:456–458

    Google Scholar 

  • De Smet I, De Boever P, Versteaete W (1998) Cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity. Br J Nutr 79:185–194

    PubMed  Google Scholar 

  • Dietschy JM (1966) Recent developments in solute and water transport across the gallbladder epithelium. Gastroenterology 50:692–707

    CAS  PubMed  Google Scholar 

  • Donnet-Hughes A, Rochat F, Serrant P, Aeschlimann JM, Schiffrin EJ (1999) Modulation of nonspecific mechanisms of defense by lactic acid bacteria: effective dose. J Dairy Sci 82:863–869

    CAS  PubMed  Google Scholar 

  • Fukushima M, Nakano M (1996) Effects of mixture of organisms, Lactobacillus acidophilus or Streptococcus faecalis on cholesterol metabolism in rats fed on a fat- and cholesterol-enriched diet. Br J Nutr 76:857–867

    CAS  PubMed  Google Scholar 

  • Fuller R (1989) Probiotics in man and animals. J Appl Bacteriol 66:365–378

    CAS  PubMed  Google Scholar 

  • Gilliland SE, Nelson CR, Maxwell C (1985) Assimilation of cholesterol by Lactobacillus acidophilus. Appl Environ Microb 49:377–381

    CAS  Google Scholar 

  • Grunewald KK (1982) Serum cholesterol levels in rats fed skim milk fermented by Lactobacillus acidophilus. J Food Sci 47:2078–2079

    CAS  Google Scholar 

  • Grunewald KK, Mitchell K (1983) Serum cholesterol levels in mice fed fermented and unfermented acidophilus milk. J Food Prot 46:315–318

    PubMed  Google Scholar 

  • Harrison VC, Peat G (1975) Serum cholesterol and bowel flora in the newborn. Am J Clin Nutr 28:1351–1355

    CAS  PubMed  Google Scholar 

  • Hashimoto H, Yamazaki K, He F, Kawase M, Hosoda M, Hosono A (1999) Hypocholesterolemic effects of Lactobacillus casei subsp. casei TMC 0409 strain observed in rats fed cholesterol contained diets. Anim Sci J 72:90–97

    Google Scholar 

  • Hepner G, Fried R, Jeor S, Fusetti St L, Morin R (1979) Hypocholesterolemic effect of yogurt and milk. Am J Clin Nutr 32:19–24

    CAS  PubMed  Google Scholar 

  • Hosono A, Tono-oka T (1995) Binding of cholesterol with lactic acid bacteria cells. Mil chwissenschaft 50:556–560

    CAS  Google Scholar 

  • Ibrahim A, El-sayed EM, El-zeini HSA, HM SFA (2005) The hypocholesterolaemic effect of milk yoghurt and soy-yoghurt containing bifidobacteria in rats fed on a cholesterol-enriched diet. Int Dairy J 15:37–44

    Google Scholar 

  • Keim NL, Marlett JA, Amundsopn CH (1981) The cholesterolemic effect of skim milk in young men consuming controlled diets. Nutr Res 1:422–429

    Google Scholar 

  • Liong MT, Shah NP (2006) Effects of a Lactobacillus casei symbiotic on serum lipoprotein, intestinal microflora, and organic acids in rats. J Dairy Sci 89:1390–1399

    CAS  PubMed  Google Scholar 

  • Liu JR, Wang SY, Chen MJ, Chen HL, Yueh PY, Lin CW (2006) Hypocholesterolaemic effects of milk-kefir and soyamilk-kefir in cholesterol-fed hamsters. Br J Nutr 95:939–946

    CAS  PubMed  Google Scholar 

  • Mann GV, Spoerry A (1974) Studies of a surfactant and cholesteremia in the Maasai. Am J Clin Nutr 27:464–469

    CAS  PubMed  Google Scholar 

  • Mikkelsen LL, Jensen BB (1998) Performance and microbial activity in gastrointestinal tract of piglets fed fermented liquid feed at weaning. J Anim Feed Sci 7:211–215

    Google Scholar 

  • Mott GE, Moore RW, Redmond HE, Reiser R (1973) Lowering of serum cholesterol by intestinal bacteria in cholesterol-fed piglets. Lipids 8:428–431

    CAS  PubMed  Google Scholar 

  • Nakajima H, Suzuki Y, Kaizu H, Hirota T (1992) Cholesterol-lowering activity of ropy fermented milk. J Food Sci 57:1327–1329

    CAS  Google Scholar 

  • Noh DO, Kim SH, Gilliland SE (1997) Incorporation of cholesterol into the cellular membrane of Lactobacillus acidophilus ATCC 43121. J Dairy Sci 80:3107–3113

    CAS  PubMed  Google Scholar 

  • Park YH, Kim JG, Shin YW, Kim HS, Kim YJ, Chun T, Kim SH, Whang KY (2008) Effects of Lactobacillus acidophilus 43121 and a mixture of Lactobacillus casei and Bifidobacterium longum on the serum cholesterol level and fecal excretion in hypercholesterolemia-induced pigs. Biosci Biotechnol Biochem 72(2):595–600

    CAS  PubMed  Google Scholar 

  • Probstfield JL, Rifkind BM (1991) The lipid research clinics coronary primary prevention trial: Design, results, and implications. Eur J Clin Pharmacol 40:S69–S75

    PubMed  Google Scholar 

  • Rao DR, Chawan CB, Pulusani SR (1981) Influence of milk and thermophilus milk on plasma cholesterol and hepatic cholesterogenesis in rats. J Food Sci 46:1339–1341

    CAS  Google Scholar 

  • Richelsen B, Kristensen K, Pedersen SB (1996) Long-term (6 months) effect of a new fermented milk product on the level of plasma 1ipoproteins—a placebo-controlled and double blind study. Eur J Clin Nutr 50:811–815

    CAS  PubMed  Google Scholar 

  • Rossouw JE, Burger E, Van der Vyver P, Ferreira JJ (1981) The effect of skim milk, yogurt and full cream on human serum lipids. Am J Clin Nutr 34:351–356

    CAS  PubMed  Google Scholar 

  • Schaafsma G, Meuling WJA, van Dokkum W, Bouley C (1998) Effects of a milk product, fermented by Lactobacillus acidophilus and with fructo-oligosaccharides added, on blood lipids in male volunteers. Eur J Clin Nutr 52:436–440

    CAS  PubMed  Google Scholar 

  • Steinmetz KA, Childs MT, Stimson C, Kushi LH, McGovern PG, Potter JD, Yarnanaka WK (1994) Effect of consumption of whole milk and skim milk on blood lipid profiles in healthy men. Am J Clin Nutr 59:612–618

    CAS  PubMed  Google Scholar 

  • St-Onge MP, Farnworth ER, Savard T, Chabot D, Mafu A, Jones PJH (2002) Kefir consumption does not alter plasma lipid levels or cholesterol fractional synthesis rates relative to milk in hyperlipidemic men: a randomized controlled trial. BMC Complement Altern Med 2:1–7

    PubMed  PubMed Central  Google Scholar 

  • Swanson KS, Grieshop CM, Flickinger EA, Bauer LL, Wolf BW, Chow JM, Garleb KA, Williams JA, Fahey GC (2002) Fructooligosaccharides and Lactobacillus acidophilus modify gut microbial populations, total tract nutrient digestibilities and fecal protein catabolite concentrations in healthy adult dogs. J Nutr 132:3721–3731

    CAS  PubMed  Google Scholar 

  • Taranto MP, Medici M, Perdigon G, Ruiz Horgado AP, Valdez GF (1998) Evidence for hypocholesterolemic effect of Lactobacillus reuteri in hypercholesterolemic mice. J Dairy Sci 81:2336–2340

    CAS  PubMed  Google Scholar 

  • Thompson LU, Jenkins DJA, Amer MA, Reichert R, Jenkins A, Kamulsky J (1982) Effect of fermented and unfermented milks on serum cholesterol. Am J Clin Nutr 36:1106–1111

    CAS  PubMed  Google Scholar 

  • Usman HA, Hosono A (2000) Effect of Administration of Lactobacillus gasseri on Serum Lipids and Fecal Steroids in Hypercholesterolemic Rats. J Dairy Sci 83:1705–1711

    CAS  PubMed  Google Scholar 

  • Van Winsen RL, Keuzenkamp D, Urlings BAP, Lipman LJA, Snijders JAM, Verheijden JHM, van Knapen F (2002) Effect of fermented feed on shedding of Enterobacteriaceae by fattening pigs. Vet Microbiol 87:267–276

    PubMed  Google Scholar 

  • Vujicic IF, Vulic M, Konyves T (1992) Assimilation of cholesterol in milk by kefir cultures. Biotechnol Lett 14:847–850

    CAS  Google Scholar 

Download references

Acknowledgment

This work was supported by a grant from the Science and Technology Supporting Project of China National Eleventh Five-Year-Plan (No. 2006BAD 04A 06).

Author information

Authors and Affiliations

  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science & Technology, Tianjin, 300457, China

    Yanping Wang, Nv Xu, Aodeng Xi, Zaheer Ahmed, Bin Zhang & Xiaojia Bai

Authors
  1. Yanping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nv Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aodeng Xi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zaheer Ahmed
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Bin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xiaojia Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yanping Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, Y., Xu, N., Xi, A. et al. Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet. Appl Microbiol Biotechnol 84, 341–347 (2009). https://doi.org/10.1007/s00253-009-2012-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00253-009-2012-x

Keywords

Search

Navigation