Abstract
Beside yeasts, lactic acid bacteria (LAB) are the most abundant microbes in must during vinification. Whereas Oenococcos oeni is commercially used as a starter culture for the biological acid reduction in wines, other species are responsible for different types of wine spoilage. Members of the genera Pediococcus, Weissella, Leuconostoc, and Lactobacillus are producers of exopolysaccharide slimes, biogenic amines, acetic acid, and other off-flavors. In order to control microbial growth, different procedures such as heating of must and addition of sulfite or lysozyme from egg white are generally applied. Yet, because of health risks, the application of sulfite should be reduced and lysozyme is not effective against all LAB. In this study, we describe exoenzymes from a Streptomyces sp. strain B578 lysing nearly all wine-relevant strains of LAB and Gram-negative acetic acid bacteria. The lytic enzymes were active under wine-making conditions, such as the presence of sulfite and ethanol, low temperatures, and low pH values. The analysis of the exoenzyme composition revealed a synergistic action of different cell wall hydrolases. In conclusion, the lytic cocktail of Streptomyces sp. B578 is a promising tool for the control of wine-spoiling bacteria.
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Acknowledgments
The authors thank Martina Schlander, Armin Gneipel (Johannes Gutenberg-Universität Mainz), as well as Liwia Rajpert (University of Warsaw, Poland) for their valuable contributions to perform this study and appreciate the financial support by the “Stiftung Rheinland-Pfalz für Innovation” and “the Research Association of the German Food Industry (FEI)”.
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This article is dedicated to Prof. Dr. Ferdinand Radler on the occasion of his 80th birthday.
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Blättel, V., Wirth, K., Claus, H. et al. A lytic enzyme cocktail from Streptomyces sp. B578 for the control of lactic and acetic acid bacteria in wine. Appl Microbiol Biotechnol 83, 839–848 (2009). https://doi.org/10.1007/s00253-009-1926-7
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DOI: https://doi.org/10.1007/s00253-009-1926-7