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From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducens

Applied Microbiology and Biotechnology volume 79, pages 11–22 (2008)Cite this article

Abstract

Mannheimia succiniciproducens is a capnophilic gram-negative bacterium isolated from bovine rumen. Wild-type M. succiniciproducens can produce succinic acid as a major fermentation product with acetic, formic, and lactic acids as byproducts during the anaerobic cultivation using several different carbon sources. Succinic acid is an important C4 building block chemical for many applications. Here, we review the progress made with M. succiniciproducens for efficient succinic acid production; the approaches taken towards the development of an integrated process for succinic acid production are described, which include strain isolation and characterization, complete genome sequencing and annotation, development of genetic tools for metabolic engineering, strain development by systems approach of integrating omics and in silico metabolic analysis, and development of fermentation and recovery processes. We also describe our current effort on further improving the performance of M. succiniciproducens and optimizing the mid- and downstream processes. Finally, we finish this mini-review by discussing the issues that need to be addressed to make this process of fermentative succinic acid production employing M. succiniciproducens to reach the industrial-scale process.

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Acknowledgments

This work was supported by the Genome-based Integrated Bioprocess Project of the Ministry of Science and Technology. Further supports by the LG Chem Chair Professorship, IBM SUR program, and Microsoft are appreciated.

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Affiliations

  1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, and Institute for the BioCentury, KAIST, Daejeon, 305-701, Republic of Korea

    Sang Yup Lee, Ji Mahn Kim, Hyohak Song, Jeong Wook Lee, Tae Yong Kim & Yu-Sin Jang

  2. Department of Bio and Brain Engineering and Bioinformatics Research Center, KAIST, Daejeon, 305-701, Republic of Korea

    Sang Yup Lee

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  1. Sang Yup Lee
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  2. Ji Mahn Kim
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  3. Hyohak Song
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  4. Jeong Wook Lee
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  5. Tae Yong Kim
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  6. Yu-Sin Jang
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Correspondence to Sang Yup Lee.

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Lee, S.Y., Kim, J.M., Song, H. et al. From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducens . Appl Microbiol Biotechnol 79, 11–22 (2008). https://doi.org/10.1007/s00253-008-1424-3

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  • DOI: https://doi.org/10.1007/s00253-008-1424-3

Keywords

  • Mannheimia succiniciproducens
  • Genome
  • Succinic acid
  • Metabolic engineering
  • Fermentation
  • Recovery