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Investigating the interaction mechanism between zinc and Saccharomyces cerevisiae using combined SEM-EDX and XAFS

Applied Microbiology and Biotechnology volume 79, pages 293–299 (2008)Cite this article

Abstract

The interaction mechanism between zinc and the intact yeast cells of Saccharomyces cerevisiae was investigated by using the scanning electron microscopy with energy-dispersive X-ray analysis, as well as X-ray absorption fine structure spectroscopy (XAFS). Displacement of H+, K+, Mg2+, and Na+ during zinc uptake confirmed the existence of both covalent interactions and ionic interactions between Zn2+ and the microbe. Ion exchange mechanism played a role in zinc uptake. The local environment of Zn accumulated in the intact yeast cells was determined by XAFS, which suggests that the nearest neighboring atom of the bound zinc ion on the biomass is oxygen atom. The adsorbed zinc ion on the intact cells of S. cerevisiae is a tetrahedron structure, with the Zn–O bond length of 1.97 Å, and the coordination number is only 3.2 of Zn–O structure in the first shell.

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Acknowledgments

The work was financially supported by the National Natural Science Foundation of China (Grant No. 50278045) and the Basic Research Fund of Tsinghua University (Grant No. JC2002054). We thank Mr. Xie Yaning and Dr. Du Yonghua (Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Beijing, China) for their help with X-ray absorption spectroscopy.

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  1. Laboratory of Environmental Technology, INET, Tsinghua University, Beijing, 100084, People’s Republic of China

    Chen Can & Wang Jianlong

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  1. Chen Can
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  2. Wang Jianlong
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Correspondence to Wang Jianlong.

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Can, C., Jianlong, W. Investigating the interaction mechanism between zinc and Saccharomyces cerevisiae using combined SEM-EDX and XAFS. Appl Microbiol Biotechnol 79, 293–299 (2008). https://doi.org/10.1007/s00253-008-1415-4

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  • DOI: https://doi.org/10.1007/s00253-008-1415-4

Keywords

  • Zn2+
  • Saccharomyces cerevisiae
  • Interaction
  • XAFS
  • Mechanism